Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-mangostin | NSC30552, a natural product, caspase-3 pathway inhibitor, performs selective inhibition of 12-LO | Homo sapiens | |
dysidenin | - |
Homo sapiens | |
michellamine B | NSC661755, potent but non-selective inhibitor, a natural anti-viral agent | Homo sapiens | |
additional information | development of an assay method for high throughput screening of libraries for platelet-type 12-hLO selective inhibitors, four organo-mercurial compounds with NCI library IDs NSC20410, NSC268879, NSC321237, and NSC321239, are also found to be selectively inhibitory, but not pursued further due to their potentially toxic side effects, overview | Homo sapiens | |
neodysidenin | natural product from marine sponge Dysidea herbacea from Papua New Guinea, extraction and purification, overview, steady-state inhibition kinetics, competitive mode of inhibition, selective for 12-LO | Homo sapiens | |
NSC172033 | a synthetic compound from the NCI library | Homo sapiens | |
NSC292213 | a synthetic compound from the NCI library | Homo sapiens | |
NSC617570 | a synthetic compound from the NCI library | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P18054 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
platelet-type 12-human lipoxygenase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
neodysidenin | pH 8.0, recombinant enzyme | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
pH 8.0, recombinant enzyme | Homo sapiens | NSC292213 | |
0.00019 | - |
pH 8.0, recombinant enzyme | Homo sapiens | NSC617570 | |
0.00021 | - |
pH 8.0, recombinant enzyme | Homo sapiens | NSC172033 | |
0.00058 | - |
pH 8.0, recombinant enzyme | Homo sapiens | alpha-mangostin | |
0.0049 | - |
pH 8.0, recombinant enzyme | Homo sapiens | michellamine B | |
0.009 | - |
above, pH 8.0, recombinant enzyme | Homo sapiens | dysidenin | |
0.1 | - |
above, pH 8.0, recombinant enzyme | Homo sapiens | neodysidenin |