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Literature summary for 1.13.11.31 extracted from
- Identification and characterization of an arachidonate 11R-lipoxygenase (2006), Arch. Biochem. Biophys., 445, 147-155.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Gersemia fruticosa |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme shows higher activity in 50 mM CAPS buffer (pH 10-11) and glycine buffer (pH 8.5-10.5) than in 50 mM Tris-HCl (pH 7-9) | Gersemia fruticosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gersemia fruticosa | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, required for activity | Gersemia fruticosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
SDS-PAGE | Gersemia fruticosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gersemia fruticosa | - |
soft coral | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11,14,17-eicosatrienoic acid + O2 | poor substrate | Gersemia fruticosa | ? | - |
? | |
| adrenic acid + O2 | - |
Gersemia fruticosa | ? | - |
? | |
| arachidonate + O2 | - |
Gersemia fruticosa | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? | |
| dihomo-gamma linolenic acid + O2 | - |
Gersemia fruticosa | ? | - |
? | |
| docosahexaenoic acid + O2 | - |
Gersemia fruticosa | 14-hydroxydocosahexaenoic acid | - |
? | |
| eicosapentaenoic acid + O2 | - |
Gersemia fruticosa | 12-hydroxyeicosapentaenoic acid | - |
? | |
| gamma linolenic acid + O2 | poor substrate | Gersemia fruticosa | 10-hydroxy-octadeca-(6Z,8E,12Z)-trienoic acid | - |
? | |
| linolenic acid + O2 | poor substrate | Gersemia fruticosa | ? | - |
? | |
| additional information | no reaction with mead acid and methyl esters of linolenic acid and arachidonate | Gersemia fruticosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 11R-lipoxygenase | - |
Gersemia fruticosa |
| 11R-LOX | - |
Gersemia fruticosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 15 | - |
Gersemia fruticosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
significant activity decrease above 25°C | Gersemia fruticosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | - |
Gersemia fruticosa |
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Release 2023.1 (January 2023)
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