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Literature summary for 1.13.11.3 extracted from

  • Guzik, U.; Hupert-Kocurek, K.; Sitnik, M.; Wojcieszylska, D.
    Protocatechuate 3,4-dioxygenase a wide substrate specificity enzyme isolated from Stenotrophomonas maltophilia KB2 as a useful tool in aromatic acid biodegradation (2014), J. Mol. Microbiol. Biotechnol., 24, 150-160 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
butanol 120.77% activity at 200 mM Stenotrophomonas maltophilia
EDTA 135.07% activity at 1 mM Stenotrophomonas maltophilia
ethanol 156.69% activity at 200 mM Stenotrophomonas maltophilia
Propanol 160.78% activity at 100 mM Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 87.62% residual activity at 3 mM Stenotrophomonas maltophilia
EDTA 18.19% residual activity at 3 mM Stenotrophomonas maltophilia
methanol 91.53% residual activity at 200 mM Stenotrophomonas maltophilia
Phenanthroline 69.69% residual activity at 3 mM Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0313
-
3,4-dihydroxybenzoate at pH 7.2 and 35°C Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxybenzoate + O2 Stenotrophomonas maltophilia 100% activity 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2 Stenotrophomonas maltophilia KB2 100% activity 3-carboxy-cis,cis-muconate
-
?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia I0DHJ0 AND I0DHJ1 alpha and beta subunit
-
Stenotrophomonas maltophilia KB2 I0DHJ0 AND I0DHJ1 alpha and beta subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + O2 23.6% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
2,4-dihydroxybenzoate + O2 43.5% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
2,5-dihydroxybenzoate + O2 33.46% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
2,6-dihydroxybenzoate + O2 30.51% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
3,4-dihydroxybenzoate + O2 100% activity Stenotrophomonas maltophilia 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2 100% activity Stenotrophomonas maltophilia KB2 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxyhydrocinnamic acid + O2 29.54% ctivity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
3,4-dihydroxyhydrocinnamic acid + O2 29.54% ctivity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia KB2 ?
-
?
3,5-dihydroxybenzoate + O2 44.33% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
4-hydroxybenzoic acid + O2
-
Stenotrophomonas maltophilia ?
-
?
4-hydroxybenzoic acid + O2
-
Stenotrophomonas maltophilia KB2 ?
-
?
caffeic acid + O2 34.21% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia ?
-
?
caffeic acid + O2 34.21% activity compared to 3,4-dihydroxybenzoate Stenotrophomonas maltophilia KB2 ?
-
?
vanillic acid + O2
-
Stenotrophomonas maltophilia ?
-
?
vanillic acid + O2
-
Stenotrophomonas maltophilia KB2 ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 22400 + 1 * 26800, calculated from amino acid sequence Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
P34O
-
Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.2
-
Stenotrophomonas maltophilia

pH Range

pH Minimum pH Maximum Comment Organism
7 11 the enzyme shows more than 50% activity between pH 7.0 and 11.0 and loses 98.3% of its original enzymatic activity at pH 4.0 and about 95.4% at pH 12.0 Stenotrophomonas maltophilia

Expression

Organism Comment Expression
Stenotrophomonas maltophilia enzyme expression is induced when cells are grown on 9 mM 4-hydroxybenzoate up