KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme exhibits Michaelis-Menten kinetics for all substrates tested | Pseudomonas oleovorans | |
0.002 | - |
gallate | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
catechol | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
L-Dopa | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
4-methylcatechol | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
caffeic acid | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
pyrogallol | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
catechin | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.014 | - |
4-Cresol | pH 7.5, 38°C | Pseudomonas oleovorans | |
0.017 | - |
3,4-dihydroxybenzoate | pH 7.5, 38°C | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
34300 | - |
trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
189900 | - |
gel filtration | Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | Pseudomonas oleovorans | - |
3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | Pseudomonas oleovorans KF707 | - |
3-carboxy-cis,cis-muconate | - |
? | |
additional information | Pseudomonas oleovorans | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | ? | - |
? | |
additional information | Pseudomonas oleovorans KF707 | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Pseudomonas oleovorans KF707 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 296.8fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
121.7 | - |
purified native enzyme, pH 7.5, 38°C | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas oleovorans | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | i.e. protocatechuate | Pseudomonas oleovorans | 3-carboxy-cis,cis-muconate | - |
? | |
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas oleovorans KF707 | 3-carboxy-cis,cis-muconate | - |
? | |
4-cresol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
caffeic acid + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
caffeic acid + O2 | - |
Pseudomonas oleovorans KF707 | ? | - |
? | |
catechin + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
catechol + O2 | reaction of EC 1.13.11.1 | Pseudomonas oleovorans | cis,cis-muconate | - |
? | |
catechol + O2 | reaction of EC 1.13.11.1 | Pseudomonas oleovorans KF707 | cis,cis-muconate | - |
? | |
gallate + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
L-DOPA + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
additional information | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | Pseudomonas oleovorans | ? | - |
? | |
additional information | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | Pseudomonas oleovorans KF707 | ? | - |
? | |
pyrogallol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
pyrogallol + O2 | - |
Pseudomonas oleovorans KF707 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Pseudomonas oleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified enzyme, 50 min, pH 7.5, relatively stable | Pseudomonas oleovorans |
70 | - |
purified enzyme, 20 min, pH 7.5, loss of 95% activity | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas oleovorans |