Literature summary for 1.13.11.3 extracted from

Frazee, R.W.; Orville, A.M.; Dolbeare, K.B.; Yu, H.; Ohlendorf, D.H.; Lipscomb, J.D.
The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: evidence for new reaction cycle intermediates (1998), Biochemistry, 37, 2131-2144.

Search for more literature for 1.13.11.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and Y447H mutant enzyme in Pseudomonas fluorescens	Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion with ammonium sulfate as the precipitant at 4°C, recombinant wild-type and Y447H mutant enzyme	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
Y447H	greatly reduced rate of protocatechuate oxygenation	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0029	-	protocatechuate	Y447H mutant enzyme	Pseudomonas putida
0.006	-	O2	Y447H mutant enzyme	Pseudomonas putida
0.029	-	protocatechuate	recombinant enzyme	Pseudomonas putida
0.03	-	protocatechuate	native enzyme	Pseudomonas putida
0.04	-	O2	native enzyme	Pseudomonas putida
0.056	-	O2	recombinant enzyme	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
no activity in Pseudomonas fluorescens	-	-	-
Pseudomonas putida	-	previously classified as Pseudomonas aeruginosa strain ATCC 23975	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
37.2	-	recombinant enzyme	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydroxybenzoate + O2	-	Pseudomonas putida	3-carboxy-cis,cis-muconate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.12	-	protocatechuate	Y447H mutant enzyme	Pseudomonas putida
68	-	protocatechuate	recombinant enzyme	Pseudomonas putida
70	-	protocatechuate	native enzyme	Pseudomonas putida

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Release 2023.1 (January 2023)