Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.005 mM, approx. 50% inhibition after 30 min, complete inhibition after 60 min, complete restoration of inactivated enzyme by addition of excess ferric EDTA complex | Rhizobium leguminosarum | |
Al3+ | EDTA salt, 0.01 mM, complete inhibition | Rhizobium leguminosarum | |
Ni2+ | EDTA salt, 0.01 mM, 72% inhibition | Rhizobium leguminosarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0175 | - |
protocatechuate | - |
Rhizobium leguminosarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 1.3-1.5 g-atom Fe3+ per mol of enzyme | Rhizobium leguminosarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE | Rhizobium leguminosarum |
29000 | - |
alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE | Rhizobium leguminosarum |
220000 | - |
gel filtration | Rhizobium leguminosarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephacryl S 300, DEAE-Sephadex A50 | Rhizobium leguminosarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | specific for protocatechuate | Rhizobium leguminosarum | 3-carboxy-cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE | Rhizobium leguminosarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Rhizobium leguminosarum |