Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | preincubation with substrate protects against inactivation | Tecoma stans | |
8-hydroxyquinoline | reversible by addition of Cu2+ | Tecoma stans | |
Ag+ | - |
Tecoma stans | |
azide | - |
Tecoma stans | |
CN- | - |
Tecoma stans | |
diethyldithiocarbamate | reversible by addition of Cu2+ | Tecoma stans | |
dithiothreitol | preincubation with substrate protects against inactivation | Tecoma stans | |
Fe2+ | - |
Tecoma stans | |
Fe3+ | - |
Tecoma stans | |
glutathione | reduced form, preincubation with substrate protects against inactivation | Tecoma stans | |
Hg2+ | - |
Tecoma stans | |
iodoacetate | preincubation with substrate protects against inactivation | Tecoma stans | |
L-cysteine | preincubation with substrate protects against inactivation | Tecoma stans | |
Mg2+ | - |
Tecoma stans | |
N-ethylmaleimide | preincubation with substrate protects against inactivation | Tecoma stans | |
Neocuproine | reversible by addition of Cu2+ | Tecoma stans | |
p-chloromercuribenzoate | preincubation with substrate protects against inactivation | Tecoma stans | |
Salicylaldoxime | reversible by addition of Cu2+ | Tecoma stans | |
Sulfide | - |
Tecoma stans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65 | - |
2,3-Dihydroxybenzoate | - |
Tecoma stans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Tecoma stans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoate + O2 | Tecoma stans | - |
2-carboxy-cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tecoma stans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Tecoma stans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Tecoma stans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxy-p-cumate + O2 | 28% of activity | Tecoma stans | 2-carboxy-4-isopropyl-cis,cis-muconate | - |
? | |
2,3-dihydroxy-p-toluate + O2 | 38% of activity | Tecoma stans | 2-carboxy-4-methyl-cis,cis-muconate | - |
? | |
2,3-dihydroxybenzoate + O2 | - |
Tecoma stans | 2-carboxy-cis,cis-muconate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Tecoma stans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | - |
cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel | Tecoma stans |
- |
- |
extremely labile, crude preparation loses 50% of activity within 3-4 h at pH 5.2 | Tecoma stans |
55 | - |
complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min | Tecoma stans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Tecoma stans |