BRENDA - Enzyme Database
show all sequences of 1.13.11.28

A new mode of ring cleavage of 2,3-dihydroxybenzoic acid in Tecoma stans (L.). Partial purification and properties of 2,3-dihydroxybenzoate 2,3-oxygenase

Sharma, H.K.; Vaidyanathan, C.S.; Eur. J. Biochem. 56, 163-171 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
preincubation with substrate protects against inactivation
Tecoma stans
8-hydroxyquinoline
reversible by addition of Cu2+
Tecoma stans
Ag+
-
Tecoma stans
azide
-
Tecoma stans
CN-
-
Tecoma stans
diethyldithiocarbamate
reversible by addition of Cu2+
Tecoma stans
dithiothreitol
preincubation with substrate protects against inactivation
Tecoma stans
Fe2+
-
Tecoma stans
Fe3+
-
Tecoma stans
glutathione
reduced form, preincubation with substrate protects against inactivation
Tecoma stans
Hg2+
-
Tecoma stans
iodoacetate
preincubation with substrate protects against inactivation
Tecoma stans
L-cysteine
preincubation with substrate protects against inactivation
Tecoma stans
Mg2+
-
Tecoma stans
N-ethylmaleimide
preincubation with substrate protects against inactivation
Tecoma stans
Neocuproine
reversible by addition of Cu2+
Tecoma stans
p-chloromercuribenzoate
preincubation with substrate protects against inactivation
Tecoma stans
Salicylaldoxime
reversible by addition of Cu2+
Tecoma stans
Sulfide
-
Tecoma stans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
65
-
2,3-Dihydroxybenzoate
-
Tecoma stans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Tecoma stans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-dihydroxybenzoate + O2
Tecoma stans
-
2-carboxy-cis,cis-muconate
-
Tecoma stans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tecoma stans
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Tecoma stans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Tecoma stans
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxy-p-cumate + O2
28% of activity
439515
Tecoma stans
2-carboxy-4-isopropyl-cis,cis-muconate
-
-
-
?
2,3-dihydroxy-p-toluate + O2
38% of activity
439515
Tecoma stans
2-carboxy-4-methyl-cis,cis-muconate
-
-
-
?
2,3-dihydroxybenzoate + O2
-
439515
Tecoma stans
2-carboxy-cis,cis-muconate
-
439515
Tecoma stans
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Tecoma stans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-20
-
cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel
Tecoma stans
-
-
extremely labile, crude preparation loses 50% of activity within 3-4 h at pH 5.2
Tecoma stans
55
-
complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min
Tecoma stans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Tecoma stans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
preincubation with substrate protects against inactivation
Tecoma stans
8-hydroxyquinoline
reversible by addition of Cu2+
Tecoma stans
Ag+
-
Tecoma stans
azide
-
Tecoma stans
CN-
-
Tecoma stans
diethyldithiocarbamate
reversible by addition of Cu2+
Tecoma stans
dithiothreitol
preincubation with substrate protects against inactivation
Tecoma stans
Fe2+
-
Tecoma stans
Fe3+
-
Tecoma stans
glutathione
reduced form, preincubation with substrate protects against inactivation
Tecoma stans
Hg2+
-
Tecoma stans
iodoacetate
preincubation with substrate protects against inactivation
Tecoma stans
L-cysteine
preincubation with substrate protects against inactivation
Tecoma stans
Mg2+
-
Tecoma stans
N-ethylmaleimide
preincubation with substrate protects against inactivation
Tecoma stans
Neocuproine
reversible by addition of Cu2+
Tecoma stans
p-chloromercuribenzoate
preincubation with substrate protects against inactivation
Tecoma stans
Salicylaldoxime
reversible by addition of Cu2+
Tecoma stans
Sulfide
-
Tecoma stans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
65
-
2,3-Dihydroxybenzoate
-
Tecoma stans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Tecoma stans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-dihydroxybenzoate + O2
Tecoma stans
-
2-carboxy-cis,cis-muconate
-
Tecoma stans
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Tecoma stans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Tecoma stans
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxy-p-cumate + O2
28% of activity
439515
Tecoma stans
2-carboxy-4-isopropyl-cis,cis-muconate
-
-
-
?
2,3-dihydroxy-p-toluate + O2
38% of activity
439515
Tecoma stans
2-carboxy-4-methyl-cis,cis-muconate
-
-
-
?
2,3-dihydroxybenzoate + O2
-
439515
Tecoma stans
2-carboxy-cis,cis-muconate
-
439515
Tecoma stans
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Tecoma stans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-20
-
cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel
Tecoma stans
-
-
extremely labile, crude preparation loses 50% of activity within 3-4 h at pH 5.2
Tecoma stans
55
-
complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min
Tecoma stans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Tecoma stans
Other publictions for EC 1.13.11.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439515
Sharma
A new mode of ring cleavage of ...
Tecoma stans
Eur. J. Biochem.
56
163-171
1975
-
-
-
-
-
-
19
1
-
1
-
1
-
3
-
-
1
-
-
1
-
-
3
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
1
-
1
-
1
-
-
-
1
-
1
-
-
3
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
439516
Sharma
-
2,3-Dihydroxybenzoate 2,3-oxyg ...
Tecoma stans
Phytochemistry
14
2135-2139
1975
-
-
-
-
-
1
20
-
2
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
20
-
-
2
-
-
1
-
-
-
-
-
1
-
-
2
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-