Cloned (Comment) | Organism |
---|---|
gene MsHPPD, DNA and amio acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative reverse transcription PCR enzyme expression analysis, promoter sequence of MsHPPD is analyzed. Recombinant overexpression in Arabidopsis thaliana in primary roots, sepals, petals, stigma, filament tubes, pollens, and the ends of seeds, no expression in in the root tip or hypocotyls | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | heterologous overexpression of gene MsHPPD in Arabidopsis thaliana significantly increases the level of beta-tocotrienol and the total vitamin E content in Arabidopsis seeds. The transgenic Arabidopsis seeds exhibit an accelerated germination time, compared with wild-type seeds under normal conditions, as well as under NaCl and abscisic acid treatments. The expression level of several genes associated with abscisic acid biosynthesis (NCED3, NCED5, and NCED9) and the abscisic acid signaling pathway (RAB18, ABI3, and ABI5) are significantly downregulated in MsHPPD-overexpressing transgenic lines, as well as the total free abscisic acid content, phenotype. MsHPPD expression in three MsHPPD-overexpressing transgenic lines and wild-type, overview. Expression levels of ABA biosynthesis and ABA signaling pathway genes in MsHPPD-overexpressing transgenic Arabidopsis thaliana | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Medicago sativa | - |
homogentisate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | A0A1S5V3J7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Medicago sativa | - |
flower | lowest expression level in early flowers | Medicago sativa | - |
leaf | high enzyme expression level, espexially in rosettes leaves | Medicago sativa | - |
additional information | expression pattern of gene MsHPPD in different organs, expression analysis. MsHPPD expression in three MsHPPD-overexpressing transgenic Arabidopsis thaliana lines and wild-type Medicago sativa | Medicago sativa | - |
root | - |
Medicago sativa | - |
seed | - |
Medicago sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Medicago sativa | homogentisate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPPD | - |
Medicago sativa |
MsHPPD | - |
Medicago sativa |
p-hydroxyphenylpyruvate dioxygenase | - |
Medicago sativa |
Organism | Comment | Expression |
---|---|---|
Medicago sativa | PEG 6000, NaCl, abscisic acid and salicylic acid significantly induce MsHPPD expression, especially in the cotyledons and root tissues | up |
General Information | Comment | Organism |
---|---|---|
malfunction | mutant plants with null alleles of HPPD are reported to exhibit a lethal photobleaching phenotype. MsHPPD expression in three MsHPPD-overexpressing transgenic lines and wild-type, overview | Medicago sativa |
metabolism | enzyme HPPD is the first committed enzyme involved in the biosynthesis of vitamin E. Vitamin E is not a single compound, but rather the collective name for a group of eight lipid-soluble antioxidants consisting of a polar chromanol head group and a hydrophobic prenyl tail, which are derived from the methylerythritol-4-phosphate (MEP) and shikimate pathways. Four of these compounds are termed tocopherols, and the other four are termed tocotrienols, and depending on the saturation level of the hydrophobic tail and also the number and position of the methyl groups on the chromanol ring, members of the vitamin E group are classified into alpha-, beta-, gamma- and delta-forms. In plants, the production of HGA is the first step in tocopherol synthesis, and homogentisic acid (HGA) is synthesized from p-hydroxyphenyl pyruvate (HPP) in a reaction catalyzed by enzyme HPPD | Medicago sativa |
physiological function | 4-hydroxyphenylpyruvate dioxygenase is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination in Medicago sativa. MsHPPD functions not only in the vitamin E biosynthetic pathway, but also plays a critical role in seed germination via affecting abscisic acid biosynthesis and signaling, overview. HPPD is essential for plant viability | Medicago sativa |