Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces avermitilis |
Protein Variants | Comment | Organism |
---|---|---|
N245I | site-directed mutagenesis, the mutant enzyme produces 13% homogentisate and 87% 4-hydroxyphenylacetate, which differs from the wild-type activity | Streptomyces avermitilis |
N245Q | site-directed mutagenesis, the mutant enzyme produces 44.5% homogentisate and 52% 4-hydroxyphenylacetate, and 4% quinolacetate, which differs from the wild-type activity | Streptomyces avermitilis |
P243T | site-directed mutagenesis, the mutant enzyme produces 13% homogentisate, 8.7% 4-hydroxyphenylacetate, and 78% quinolacetate, which differs from the wild-type activity | Streptomyces avermitilis |
S230A | site-directed mutagenesis, the mutant enzyme produces 11.6% homogentisate, 30.6% 4-hydroxyphenylacetate, and 57.8% quinolacetate, which differs from the wild-type activity | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant P243T | Streptomyces avermitilis | |
0.015 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant N245Q | Streptomyces avermitilis | |
0.027 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, wild-type enzyme and mutant N245I | Streptomyces avermitilis | |
0.047 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant S230A | Streptomyces avermitilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Streptomyces avermitilis | - |
homogentisate + CO2 + 4-hydroxyphenylacetate | the wild-type enzyme produces 85% homogentisate and 15% 4-hydroxyphenylacetate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Streptomyces avermitilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 | reaction mechanism, overview | Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Streptomyces avermitilis | homogentisate + CO2 + 4-hydroxyphenylacetate | the wild-type enzyme produces 85% homogentisate and 15% 4-hydroxyphenylacetate | ? |
Synonyms | Comment | Organism |
---|---|---|
HPPD | - |
Streptomyces avermitilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant N245Q | Streptomyces avermitilis | |
0.64 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant N245I | Streptomyces avermitilis | |
2.1 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant P243T | Streptomyces avermitilis | |
2.8 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant S230A | Streptomyces avermitilis | |
6.8 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, wild-type enzyme | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces avermitilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant N245Q | Streptomyces avermitilis | |
24 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant N245I | Streptomyces avermitilis | |
60 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant S230A | Streptomyces avermitilis | |
241 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, mutant P243T | Streptomyces avermitilis | |
254 | - |
4-hydroxyphenylpyruvate | pH 7.0, 25°C, wild-type enzyme | Streptomyces avermitilis |