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Literature summary for 1.13.11.27 extracted from
- Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and Evidence for the proposed benzene oxide intermediate in homogentisate formation (2004), Biochemistry, 43, 663-674.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required for activity | Streptomyces avermitilis |  |
| catalase | from bovine liver, required for activity | Streptomyces avermitilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces avermitilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F337I | 0.67 of wild-type activity | Streptomyces avermitilis |
| F341Y | 16.9% of wild-type activity | Streptomyces avermitilis |
| N216I | 0.15% of wild-type activity | Streptomyces avermitilis |
| N216I/F337I | inactive | Streptomyces avermitilis |
| N216I/F337I/F341Y | inactive | Streptomyces avermitilis |
| N216I/F341Y | inactive | Streptomyces avermitilis |
| P214T | 1.6% of wild-type activity | Streptomyces avermitilis |
| P214T/F337I/F341Y | inactive | Streptomyces avermitilis |
| P214T/N216I | inactive | Streptomyces avermitilis |
| P214T/N216I/F337I | inactive | Streptomyces avermitilis |
| P214T/N216I/F337I/F341Y | inactive | Streptomyces avermitilis |
| P214T/N216I/F341Y | inactive | Streptomyces avermitilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity | Streptomyces avermitilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + O2 | Streptomyces avermitilis | - |
homogentisate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q53586 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + O2 | - |
Streptomyces avermitilis | homogentisate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPD | - |
Streptomyces avermitilis |
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Release 2023.1 (January 2023)
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