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Literature summary for 1.13.11.27 extracted from

  • Roche, P.A.; Moorehead, T.J.; Hamilton, G.A.
    Purification and properties of hog liver 4-hydroxyphenylpyruvate dioxygenase (1982), Arch. Biochem. Biophys., 216, 62-73.
    View publication on PubMed
Search for more literature for 1.13.11.27

Activating Compound

Activating Compound Comment Organism Structure
1-butanol 5% v/v, 2.9fold activation Sus scrofa
1-propanol 10% v/v, 2.1fold activation Sus scrofa
acetone 10% v/v, 5.7fold activation Sus scrofa
acetonitrile 10% v/v, 3.6fold activation Sus scrofa
Cyclohexanol 5% v/v, 4.4fold activation Sus scrofa
dioxane 10% v/v, 2.8fold activation Sus scrofa
ethanol 10% v/v, 2fold activation Sus scrofa
ether 10% v/v, 2.6fold activation Sus scrofa
methanol 10% v/v, 1.6fold activation Sus scrofa
methyl-cellosolve 10% v/v, 2.2fold activation Sus scrofa
tetrahydrofuran 10% v/v, 6.7fold activation Sus scrofa

General Stability

General Stability Organism
stable to freezing and thawing Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the Km-value is the same at 25°C and at 38°C Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
copper 0.4 atoms of Fe per 89000 Da enzyme. Removal of copper does not correlate with loss of activity Sus scrofa
Iron 0.9 atoms of Fe per 89000 Da enzyme. Removal of iron correlates with loss of activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa
50000 54000 gel filtration Sus scrofa
89000
-
 equilibrium ultracentrifugation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 hog
-

Oxidation Stability

Oxidation Stability Organism
routinely stored in presence of O2 Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.6
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, concentrated solution, 1 mg/ml, pH 6.0, stable for 6 months or more Sus scrofa
-20°C, on long-term storage the enzyme forms polymers, reversal by thiols Sus scrofa
4°C, dilute solutions, 0.1 mg/ml, 100 mM sodium acetate buffer, PH 6.0, stable for up to 2 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
 Sus scrofa homogentisate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 enzymatic rate increases approximately 5fold for every 10°C increase, Km-value is the same at 25°C and at 38°C Sus scrofa