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Literature summary for 1.13.11.27 extracted from

  • Buckthal, D.J.; Roche, P.A.; Moorehead, T.J.; Forbes, B.J.R.; Hamilton, G.A.
    4-Hydroxyphenylpyruvate dioxygenase from pig liver (1987), Methods Enzymol., 142, 132-138.
    View publication on PubMed

General Stability

General Stability Organism
long-term storage of concentrated solutions in presence of air leads to polymerization Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the Km-value is the same at 25°C and at 38°C Sus scrofa
0.04
-
4-hydroxyphenylpyruvate
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
copper 0.4 atoms of Fe per 89000 Da enzyme. Removal of copper does not correlate with loss of activity Sus scrofa
Iron 0.9 atoms of Fe per 89000 Da enzyme. Removal of iron correlates with loss of activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa
89000
-
method not mentioned Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylpyruvate + O2 Sus scrofa enzyme participates in catabolism of tyrosine homogentisate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 rate-determining step in catalysis is a protein conformation change Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, concentrated enzyme solution, 1.0 mg/ml, stable for 6 months or more Sus scrofa
4°C, 10 mM sodium acetate buffer, pH 6.0, little loss of activity after 2 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2 best substrate Sus scrofa homogentisate + CO2
-
?
4-hydroxyphenylpyruvate + O2 enzyme participates in catabolism of tyrosine Sus scrofa homogentisate + CO2
-
?
phenylpyruvate + O2 at 10% of the activity with 4-hydroxyphenylpyruvate Sus scrofa 2-hydroxyphenylacetate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
enzymatic rate increases approximately 5fold for every 10°C increase, Km-value is the same at 25°C and at 38°C Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5 7 reaction rate is constant between pH 5 and pH 7 Sus scrofa