General Stability | Organism |
---|---|
long-term storage of concentrated solutions in presence of air leads to polymerization | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value is the same at 25°C and at 38°C | Sus scrofa | |
0.04 | - |
4-hydroxyphenylpyruvate | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 0.4 atoms of Fe per 89000 Da enzyme. Removal of copper does not correlate with loss of activity | Sus scrofa | |
Iron | 0.9 atoms of Fe per 89000 Da enzyme. Removal of iron correlates with loss of activity | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, nonidentical subunits, SDS-PAGE | Sus scrofa |
89000 | - |
method not mentioned | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Sus scrofa | enzyme participates in catabolism of tyrosine | homogentisate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 | rate-determining step in catalysis is a protein conformation change | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, concentrated enzyme solution, 1.0 mg/ml, stable for 6 months or more | Sus scrofa |
4°C, 10 mM sodium acetate buffer, pH 6.0, little loss of activity after 2 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | best substrate | Sus scrofa | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | enzyme participates in catabolism of tyrosine | Sus scrofa | homogentisate + CO2 | - |
? | |
phenylpyruvate + O2 | at 10% of the activity with 4-hydroxyphenylpyruvate | Sus scrofa | 2-hydroxyphenylacetate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, nonidentical subunits, SDS-PAGE | Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzymatic rate increases approximately 5fold for every 10°C increase, Km-value is the same at 25°C and at 38°C | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | reaction rate is constant between pH 5 and pH 7 | Sus scrofa |