Cloned (Comment) | Organism |
---|---|
- |
Penicillium olsonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1H-2-benzyl-3-hydroxy-4-oxoquinolin | competitive | Penicillium olsonii | |
diethyldithiocarbamate | 100 nM, complete inhibition | Penicillium olsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
kaempferol | pH 6, 25°C | Penicillium olsonii | |
0.019 | - |
quercetin | pH 6, 25°C | Penicillium olsonii | |
0.02 | - |
galangin | pH 6, 25°C | Penicillium olsonii | |
0.085 | - |
fisetin | pH 6, 25°C | Penicillium olsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | contains 0.9 copper atoms per protein | Penicillium olsonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, SDS-PAGE | Penicillium olsonii |
56000 | - |
gel filtration | Penicillium olsonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium olsonii | A7Y9J1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the purified enzyme is a mixture of at least four glycoforms | Penicillium olsonii |
Purification (Comment) | Organism |
---|---|
- |
Penicillium olsonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:rutin-grown cell | - |
Penicillium olsonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
175 | - |
- |
Penicillium olsonii |
Storage Stability | Organism |
---|---|
4°C, 6 months, the enzyme retains 81% of its initial activity | Penicillium olsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fisetin + O2 | 16.8% of the activity with quercetin | Penicillium olsonii | ? | - |
? | |
galangin + O2 | 21% of the activity with quercetin | Penicillium olsonii | ? | - |
? | |
kaempferol + O2 | activity is 2.18fold higher than with quercetin | Penicillium olsonii | ? | - |
? | |
additional information | flavonol, morin, 3,6-dihydroxyflavone and 3,7-dihydroxyflavone are transformed at a rate of less than 1% of that found for quercetin | Penicillium olsonii | ? | - |
? | |
quercetin + O2 | - |
Penicillium olsonii | 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Penicillium olsonii |
Synonyms | Comment | Organism |
---|---|---|
quercetinase | - |
Penicillium olsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
quercetin oxidation | Penicillium olsonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.3 | - |
galangin | pH 6, 25°C | Penicillium olsonii | |
56.7 | - |
fisetin | pH 6, 25°C | Penicillium olsonii | |
166.7 | - |
quercetin | pH 6, 25°C | Penicillium olsonii | |
466.7 | - |
kaempferol | pH 6, 25°C | Penicillium olsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
0.1 M MES buffer | Penicillium olsonii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
1H-2-benzyl-3-hydroxy-4-oxoquinolin | pH 6, 25°C | Penicillium olsonii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penicillium olsonii | isoelectric focusing analysis of the purified enzyme reveals some heterogeneity since two major bands with apparent pI of 5.81 and 6.07 as well as two minor bands with apparent pI of 5.55 and 6.38 are observed. The purified enzyme is a mixture of at least four glycoforms | - |
additional information |