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Literature summary for 1.13.11.20 extracted from

  • Yamaguchi, K.; Hosokawa, Y.; Kohashi, N.; Kori, Y.; Sakakibara, S.; Ueda, I.
    Rat liver cysteine dioxygenase (cysteine oxidase). Further purification, characterization, and analysis of the activation and inactivation (1978), J. Biochem., 83, 479-491.
    View publication on PubMed
Search for more literature for 1.13.11.20

Activating Compound

Activating Compound Comment Organism Structure
cysteamine
-
 Rattus norvegicus
diethyldithiocarbamate without protein-A: 30% activation at 0.1 mM Rattus norvegicus
DL-homocysteine
-
 Rattus norvegicus
L-cysteine activates the purified enzyme under anaerobic conditions Rattus norvegicus
N-acetyl-L-cysteine
-
 Rattus norvegicus
Neocuproine without protein-A: slight activation at 0.1 mM Rattus norvegicus
S-methyl-L-cysteine
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl with protein-A: 100% inhibition at 0.1 mM, without protein-A: 58% inhibition at 0.1 mM Rattus norvegicus
8-hydroxyquinoline with protein-A: 100% inhibition at 0.1 mM, without protein-A: 99% inhibition at 0.1 mM Rattus norvegicus
Bathocuproine sulfonate with protein-A: 5% inhibition at 0.1 mM, without protein-A: 38% inhibition at 0.1 mM Rattus norvegicus
bathophenanthroline sulfonate with protein-A: 88% inhibition, without protein-A: 100% inhibition Rattus norvegicus
Carboxyethyl-L-cysteine 53% inhibition at 1 mM Rattus norvegicus
carboxymethyl-L-cysteine 37% inhibition at 1 mM Rattus norvegicus
cysteamine 39% inhibition at 10 mM Rattus norvegicus
D-cysteine 47% inhibition at 1 mM Rattus norvegicus
diethyldithiocarbamate with protein-A: not inhibitory at 0.1 mM Rattus norvegicus
DL-homocysteine 87% inhibition at 10 mM Rattus norvegicus
EDTA with protein-A: 97% inhibition at 0.1 mM, with protein-A: 99% inhibition at 0.1 mM Rattus norvegicus
EGTA with protein-A: 100% inhibition at 0.1 mM, without protein-A: 95% inhibition at 0.1 mM Rattus norvegicus
N-acetyl-L-cysteine 35% inhibition at 10 mM Rattus norvegicus
Neocuproine with protein-A: 18% inhibition at 0.1 mM, without protein-A: slight activation at 0.1 mM Rattus norvegicus
o-phenanthroline without protein-A: 96% inhibition at 0.1 mM, with protein A: 100% inhibition at 0.1 mM Rattus norvegicus
S-methyl-L-cysteine 34% inhibition at 1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 cysteine in the presence of protein-A Rattus norvegicus
0.67
-
 cysteine in the absence of protein-A Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains 1 atom of Fe per mol of enzyme Rattus norvegicus
Fe Fe2+ required, 0.8-0.9 gatom of iron per 22500 g of purified protein Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
 1 * 22500, SDS-PAGE Rattus norvegicus
23000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid and irreversible inactivation under aerobic conditions, inactivation can be prevented by a distinct cytoplasmic protein, i.e. protein A Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
using acetone precipitation, first chromatography on DEAE-cellulose column, second chromatography on DEAE-cellulose column, chromatography on Sephadex G-100 column, hydroxyapatite column, DEAE-Sephadex A-25 column and Sephadex G-75 column Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, no significant loss of activity after 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + O2
-
 Rattus norvegicus 3-sulfino-L-alanine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 22500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 for the anaerobic activation of purified enzyme by L-cysteine Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information protein-A added to the assay mixture Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 D-cysteine
-
 Rattus norvegicus
0.75
-
 S-methyl-L-cysteine
-
 Rattus norvegicus
1.5
-
 cysteamine
-
 Rattus norvegicus