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Literature summary for 1.13.11.20 extracted from

  • Sakakibara, S.; Yamaguchi, K.; Hosokawa, Y.; Kohashi, N.; Ueda, I.; Sakamoto, Y.
    Purification and some properties of rat liver cysteine oxidase (cysteine dioxygenase) (1976), Biochim. Biophys. Acta, 422, 273-279.
    View publication on PubMed
Search for more literature for 1.13.11.20

Activating Compound

Activating Compound Comment Organism Structure
2,2'-dipyridyl slight activation at 0.01 mM Rattus norvegicus
8-hydroxyquinoline slight activation at 0.01 mM Rattus norvegicus
L-cysteine activates the purified enzyme under anaerobic conditions Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 92% inhibition at 0.1 mM Rattus norvegicus
8-hydroxyquinoline 59% inhibition at 0.1 mM Rattus norvegicus
Bathocuproine sulfonate 80% inhibition at 0.01 mM, 83% inhibition at 0.1 mM Rattus norvegicus
bathophenanthroline sulfonate 68% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
Cu2+ 90% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
cystine 42% inhibition at 5 mM Rattus norvegicus
D-cysteine 20% inhibition at 10 mM Rattus norvegicus
diethyldithiocarbamate no significant inhibition Rattus norvegicus
DL-homocysteine 47% inhibition at 10 mM Rattus norvegicus
DL-homocystine 47% inhibition at 5 mM Rattus norvegicus
EDTA 59% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
Fe2+ 50% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
o-phenanthroline 13% inhibition at 0.01 mM, 88% inhibition at 0.1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 L-cysteine
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe prosthetic group Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid and irreversible inactivation under aerobic conditions, inactivation can be prevented by a distinct cytoplasmic protein, i.e. protein A Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, ammonium sulfate fractionation and column chromatography on DEAE-cellulose, Sephadex G-200 and Sephadex G-100 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + O2 highly specific for L-cysteine Rattus norvegicus 3-sulfino-L-alanine
-
 ?
additional information L-cystine, D-cysteine, DL-homocysteine and cysteamine do not serve as substrates Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38 40 for the anaerobic activation of the purified enzyme by L-cysteine Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 9.5 for the anaerobic activation of the purified enzyme by L-cysteine Rattus norvegicus
8.5 9 purified enzyme, of enzyme reaction Rattus norvegicus