Literature summary for 1.13.11.2 extracted from

Zeng, X.H.; Du, H.; Zhao, H.M.; Xiang, L.; Feng, N.X.; Li, H.; Li, Y.W.; Cai, Q.Y.; Mo, C.H.; Wong, M.H.; He, Z.L.
Insights into the binding interaction of substrate with catechol 2,3-dioxygenase from biophysics point of view (2020), J. Hazard. Mater., 391, 122211 .

Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 (DE3)	Rhodococcus sp. 2G

Protein Variants

Protein Variants	Comment	Organism
E270A	mutant enzyme is almost completely inactivated	Rhodococcus sp. 2G
H157A	mutant enzyme is almost completely inactivated	Rhodococcus sp. 2G
H202A	mutant enzyme is almost completely inactivated	Rhodococcus sp. 2G
H219A	mutant enzyme is almost completely inactivated	Rhodococcus sp. 2G
T254A	catalytic activity of the mutant enzyme hardly changes. It shows a broader substrate specificity than the wild-type enzyme. The activities of the mutant enzyme on 3-methylcatechol and 4-methylcatechol increases 3.7fold and 2.5folds, respectively, as compared to catechol	Rhodococcus sp. 2G

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03935	-	catechol	pH 7.5, 30°C	Rhodococcus sp. 2G

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	calculated from sequence	Rhodococcus sp. 2G
47000	-	SDS-PAGE	Rhodococcus sp. 2G

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
catechol + O2	Rhodococcus sp. 2G	-	cis,cis-muconate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus sp. 2G	A0A1J0TVF4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodococcus sp. 2G

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-methylcatechol + O2	wild-type enzyme shows 6.1% of the activity as compared to catechol. The activities of the mutant enzyme T254A increases 3.7fold as compared to catechol	Rhodococcus sp. 2G	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
4-chlorocatechol + O2	19.2% of the activity as compared to catechol	Rhodococcus sp. 2G	5-chloro-2-hydroxy-6-oxohexa-2,4-dienoate	-	?
4-methylcatechol + O2	wild-type enzyme shows 92.1% of the activity as compared to catechol. The activities of the mutant enzyme T254A increases 2.5fold as compared to catechol	Rhodococcus sp. 2G	2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate	-	?
catechol + O2	-	Rhodococcus sp. 2G	cis,cis-muconate	-	?
catechol + O2	-	Rhodococcus sp. 2G	2-hydroxymuconate-6-semialdehyde	-	?

Synonyms

Synonyms	Comment	Organism
C23O-2G	-	Rhodococcus sp. 2G

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Rhodococcus sp. 2G

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Rhodococcus sp. 2G

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Release 2023.1 (January 2023)