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Literature summary for 1.13.11.2 extracted from

  • Hupert-Kocurek, K.; Wojcieszylska, D.; Guzik, U.; Borowski, T.
    A single amino acid substitution within catalytically non-active N-terminal domain of catechol 2,3-dioxygenase (C23O) increases enzyme activity towards 4-chlorocatechol (2015), J. Mol. Catal. B, 122, 64-71 .
No PubMed abstract available
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Planococcus sp. S5

Protein Variants

Protein Variants Comment Organism
M65T the mutant show increased activity compared to the wild type enzyme Planococcus sp. S5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Planococcus sp. S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Planococcus sp. S5 E7DDG2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00125
-
 wild type enzyme, with 3-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.00155
-
 mutant enzyme M65T, with 3-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.00938
-
 wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.02065
-
 wild type enzyme, with catechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.0214
-
 wild type enzyme, with 4-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.02245
-
 mutant enzyme M65T, with catechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.0312
-
 mutant enzyme M65T, with 4-methylcatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5
0.04843
-
 mutant enzyme M65T, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C Planococcus sp. S5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 6.12% activity compared to catechol Planococcus sp. S5 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-chlorocatechol + O2 44.88% activity compared to catechol Planococcus sp. S5 4-chloro-2-hydroxymuconate semialdehyde
-
 ?
4-methylcatechol + O2 101.91% activity compared to catechol Planococcus sp. S5 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2
-
 Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2 100% activity Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
C23o
-
 Planococcus sp. S5

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Planococcus sp. S5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Planococcus sp. S5