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Literature summary for 1.13.11.2 extracted from

  • Takeo, M.; Nishimura, M.; Shirai, M.; Takahashi, H.; Negoro, S.
    Purification and characterization of catechol 2,3-dioxygenase from the aniline degradation pathway of Acinetobacter sp. YAA and its mutant enzyme, which resists substrate inhibition (2007), Biosci. Biotechnol. Biochem., 71, 1668-1675.
    View publication on PubMed
Search for more literature for 1.13.11.2

Protein Variants

Protein Variants Comment Organism
E286A mutation results in reduction in activity Acinetobacter sp.
E286D activity is similar to wild-type enzyme Acinetobacter sp.
E286K specific activity is 2.4fold higher than wild-type activity, in contrast with wild-type enzyme mutant enzyme shows no substrate inhibition Acinetobacter sp.
E286R activity is similar to wild-type enzyme Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
catechol wild-type enzyme: substrate inhibition. Mutant enzyme E286K: no substrate inhibition Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00147
-
 catechol 25°C, pH 7.2, wild-type enzyme Acinetobacter sp.
0.00248
-
 catechol 25°C, pH 7.2, mutant enzyme E286K Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, SDS-PAGE Acinetobacter sp.
147000
-
 gel filtration Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
 recombinantly expressed in Escherichia coli JM109
-
Acinetobacter sp. YAA
-
 recombinantly expressed in Escherichia coli JM109
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenyl + O2 5% of the activity with catechol Acinetobacter sp. ?
-
 ?
2,3-dihydroxybiphenyl + O2 5% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
3-methylcatechol + O2 19% of the activity with catechol Acinetobacter sp. 2-hydroxy-3-methylmuconate semialdehyde
-
 ?
3-methylcatechol + O2 19% of the activity with catechol Acinetobacter sp. YAA 2-hydroxy-3-methylmuconate semialdehyde
-
 ?
4-chlorocatechol + O2 46% of the activity with catechol Acinetobacter sp. ?
-
 ?
4-chlorocatechol + O2 46% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
4-methylcatechol + O2 57% of the activity with catechol Acinetobacter sp. ?
-
 ?
4-methylcatechol + O2 57% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
catechol + O2
-
 Acinetobacter sp. 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2
-
 Acinetobacter sp. YAA 2-hydroxymuconate semialdehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 35000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
AtdB
-
 Acinetobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.3
-
 catechol 25°C, pH 7.2, wild-type enzyme Acinetobacter sp.
34.7
-
 catechol 25°C, pH 7.2, mutant enzyme E286K Acinetobacter sp.