Protein Variants | Comment | Organism |
---|---|---|
E286A | mutation results in reduction in activity | Acinetobacter sp. |
E286D | activity is similar to wild-type enzyme | Acinetobacter sp. |
E286K | specific activity is 2.4fold higher than wild-type activity, in contrast with wild-type enzyme mutant enzyme shows no substrate inhibition | Acinetobacter sp. |
E286R | activity is similar to wild-type enzyme | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catechol | wild-type enzyme: substrate inhibition. Mutant enzyme E286K: no substrate inhibition | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00147 | - |
catechol | 25°C, pH 7.2, wild-type enzyme | Acinetobacter sp. | |
0.00248 | - |
catechol | 25°C, pH 7.2, mutant enzyme E286K | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Acinetobacter sp. |
147000 | - |
gel filtration | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
recombinantly expressed in Escherichia coli JM109 | - |
Acinetobacter sp. YAA | - |
recombinantly expressed in Escherichia coli JM109 | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybiphenyl + O2 | 5% of the activity with catechol | Acinetobacter sp. | ? | - |
? | |
2,3-dihydroxybiphenyl + O2 | 5% of the activity with catechol | Acinetobacter sp. YAA | ? | - |
? | |
3-methylcatechol + O2 | 19% of the activity with catechol | Acinetobacter sp. | 2-hydroxy-3-methylmuconate semialdehyde | - |
? | |
3-methylcatechol + O2 | 19% of the activity with catechol | Acinetobacter sp. YAA | 2-hydroxy-3-methylmuconate semialdehyde | - |
? | |
4-chlorocatechol + O2 | 46% of the activity with catechol | Acinetobacter sp. | ? | - |
? | |
4-chlorocatechol + O2 | 46% of the activity with catechol | Acinetobacter sp. YAA | ? | - |
? | |
4-methylcatechol + O2 | 57% of the activity with catechol | Acinetobacter sp. | ? | - |
? | |
4-methylcatechol + O2 | 57% of the activity with catechol | Acinetobacter sp. YAA | ? | - |
? | |
catechol + O2 | - |
Acinetobacter sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Acinetobacter sp. YAA | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
AtdB | - |
Acinetobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.3 | - |
catechol | 25°C, pH 7.2, wild-type enzyme | Acinetobacter sp. | |
34.7 | - |
catechol | 25°C, pH 7.2, mutant enzyme E286K | Acinetobacter sp. |