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Literature summary for 1.13.11.2 extracted from

  • Junca, H.; Plumeier, I.; Hecht, H.J.; Pieper, D.H.
    Difference in kinetic behaviour of catechol 2,3-dioxygenase variants from a polluted environment (2004), Microbiology, 150, 4181-4187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
4-Chlorocatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
0.0011
-
catechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
0.0015
-
3-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
0.0016
-
4-Chlorocatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
0.0019
-
4-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
0.0025
-
catechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
0.0032
-
3-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
0.0052
-
4-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylcatechol + O2 Pseudomonas sp. degradation of aromatic compounds ?
-
?
catechol + O2 Pseudomonas sp. degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q83U22 strains 1YB2 and 1YdBTEX2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-3-methylmuconate semialdehyde
-
?
4-chlorocatechol + O2
-
Pseudomonas sp. 4-chloro-2-hydroxymuconate semialdehyde
-
?
4-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-4-methylmuconate semialdehyde
-
?
alkylcatechol + O2 degradation of aromatic compounds Pseudomonas sp. ?
-
?
catechol + O2
-
Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 degradation of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
3-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
17
-
4-Chlorocatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
25
-
3-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
38
-
catechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
45
-
4-Chlorocatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
53
-
4-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
64
-
4-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
128
-
catechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.