Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
H199N | strongly reduced activity, significant changes in pH profile | Pseudomonas stutzeri |
H246A | strongly reduced activity, significant changes in pH profile | Pseudomonas stutzeri |
H246N | strongly reduced activity, significant changes in pH profile | Pseudomonas stutzeri |
Y255F | strongly reduced activity | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | partially reversible by addition of Fe(NH4)2(SO4)2 | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
catechol | wild type enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
0.005 | - |
catechol | Y255F mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
0.434 | - |
catechol | H199N mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
0.714 | - |
catechol | H246N mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Pseudomonas stutzeri |
35100 | - |
calculated from deduced amino acid sequence | Pseudomonas stutzeri |
35110 | - |
mass sprectrometry | Pseudomonas stutzeri |
117000 | - |
gel filtration | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas stutzeri | - |
2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas stutzeri OX1 | - |
2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | Q8KLV4 | - |
- |
Pseudomonas stutzeri OX1 | Q8KLV4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methoxycatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri | 2-hydroxy-3-methoxymuconate semialdehyde | - |
? | |
3-methoxycatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri OX1 | 2-hydroxy-3-methoxymuconate semialdehyde | - |
? | |
4-ethylcatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri | 4-ethyl-2-hydroxymuconate semialdehyde | - |
? | |
4-ethylcatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri OX1 | 4-ethyl-2-hydroxymuconate semialdehyde | - |
? | |
4-formylcatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri | 4-formyl-2-hydroxymuconate semialdehyde | - |
? | |
4-formylcatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri OX1 | 4-formyl-2-hydroxymuconate semialdehyde | - |
? | |
4-methylcatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri | 2-hydroxy-4-methylmuconate semialdehyde | - |
? | |
4-nitrocatechol + O2 | rapid inactivation of enzyme during turnover | Pseudomonas stutzeri | 2-hydroxy-4-nitromuconate semialdehyde | - |
? | |
catechol + O2 | - |
Pseudomonas stutzeri | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | most effective substrate | Pseudomonas stutzeri | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Pseudomonas stutzeri OX1 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | most effective substrate | Pseudomonas stutzeri OX1 | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE, mass spectrometry | Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
catechol | H199N mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
1.6 | - |
catechol | H246N mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
8.8 | - |
catechol | Y255F mutant enzyme, pH 7.5, 25°C | Pseudomonas stutzeri | |
200 | - |
catechol | wild type enzyme, pH 7.5, 25°C | Pseudomonas stutzeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
rapid loss of activity below pH 6, very slow decrease of activity above pH 6 | Pseudomonas stutzeri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
rapid loss of activity below pH 6 | Pseudomonas stutzeri |