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Literature summary for 1.13.11.2 extracted from

  • Milo, R.E.; Duffner, F.M.; Muller, R.
    Catechol 2,3-dioxygenase from the thermophilic, phenol-degrading Bacillus thermoleovorans strain A2 has unexpected low thermal stability (1999), Extremophiles, 3, 185-190.
    View publication on PubMed
Search for more literature for 1.13.11.2

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli
-
 Geobacillus thermoleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ one iron per enzyme subunit, AAS, iron is tightly bound Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34700
-
 4 * 34700, SDS-PAGE Geobacillus thermoleovorans
135000
-
 gel filtration Geobacillus thermoleovorans

Organic Solvent Stability

Organic Solvent Comment Organism
additional information Tris-HCl, inhibitory effect on the activity Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Geobacillus thermoleovorans
-
-
-
Geobacillus thermoleovorans A2
-
-
-

Oxidation Stability

Oxidation Stability Organism
addition of H2O2 to the enzyme completely destroyes activity Geobacillus thermoleovorans

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorocatechol + O2 weak Geobacillus thermoleovorans 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
3-chlorocatechol + O2 weak Geobacillus thermoleovorans A2 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2 weak Geobacillus thermoleovorans 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2 weak Geobacillus thermoleovorans A2 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2 weak Geobacillus thermoleovorans 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2 weak Geobacillus thermoleovorans A2 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2
-
 Escherichia coli 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2
-
 Geobacillus thermoleovorans 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2
-
 Geobacillus thermoleovorans A2 2-hydroxymuconate semialdehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 34700, SDS-PAGE Geobacillus thermoleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1.5 min, 50% loss of original activity under aerobic conditions Geobacillus thermoleovorans
70
-
 10 min, 50% loss of original activity under nitrogen atmosphere Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Geobacillus thermoleovorans