Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | one iron per enzyme subunit, AAS, iron is tightly bound | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34700 | - |
4 * 34700, SDS-PAGE | Geobacillus thermoleovorans |
135000 | - |
gel filtration | Geobacillus thermoleovorans |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | Tris-HCl, inhibitory effect on the activity | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Geobacillus thermoleovorans | - |
- |
- |
Geobacillus thermoleovorans A2 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
addition of H2O2 to the enzyme completely destroyes activity | Geobacillus thermoleovorans |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorocatechol + O2 | weak | Geobacillus thermoleovorans | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3-chlorocatechol + O2 | weak | Geobacillus thermoleovorans A2 | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Escherichia coli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | weak | Geobacillus thermoleovorans | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | weak | Geobacillus thermoleovorans A2 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Escherichia coli | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | weak | Geobacillus thermoleovorans | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | weak | Geobacillus thermoleovorans A2 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Escherichia coli | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Geobacillus thermoleovorans | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Geobacillus thermoleovorans A2 | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34700, SDS-PAGE | Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1.5 min, 50% loss of original activity under aerobic conditions | Geobacillus thermoleovorans |
70 | - |
10 min, 50% loss of original activity under nitrogen atmosphere | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Geobacillus thermoleovorans |