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Literature summary for 1.13.11.2 extracted from

  • Kojima, Y.; Itada, N.; Hayaishi, O.
    Metapyrocatechase: a new catechol-cleaving enzyme (1961), J. Biol. Chem., 236, 2223-2228.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
p-chloromercuribenzoate not Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not: Co2+, Mn2+, Cu2+, Cr3+, Zn2+, Ni2+, Cd2+, Ca2+ Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
OC1
-
Pseudomonas sp. OC1
-
OC1
-

Oxidation Stability

Oxidation Stability Organism
extremely sensitive to O2, easily inactivated in presence of air Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-10°C, 1 week, under nitrogen gas, 72% of the orginal activity Pseudomonas sp.
0°C, 4 h, about 30% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2 highly specific for Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 highly specific for Pseudomonas sp. OC1 2-hydroxymuconate semialdehyde
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pseudomonas sp.