Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | - |
Pseudomonas putida | |
H2O2 | strong inactivation | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 4-5 gatom of non-heme iron per mol of protein | Pseudomonas putida | |
Iron | the non-heme bound-iron is in the ferrous state and is essential for activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Pseudomonas putida |
138000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoic acid + O2 | 0.125% of the activity with 3,4-dihydroxyphenylacetate | Pseudomonas putida | 2-hydroxy-5-carboxymuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Pseudomonas putida | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylpropionate + O2 | 2.86% of the activity with 3,4-dihydroxyphenylacetate | Pseudomonas putida | 2-hydroxy-5-carboxyethylmuconate semialdehyde | - |
? | |
3-(3,4-dihydroxyphenyl)-DL-alanine + O2 | - |
Pseudomonas putida | (2E,4Z)-7-amino-5-formyl-2-hydroxyocta-2,4-dienedioic acid | - |
? | |
additional information | very low activity with various 4-substituted catechols | Pseudomonas putida | ? | - |
? | |
N-formyl-3-(3,4-dihydroxyphenyl)-DL-alanine + O2 | - |
Pseudomonas putida | (2E,4Z)-5-formyl-7-(formylamino)-2-hydroxyocta-2,4-dienedioic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Pseudomonas putida |