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Literature summary for 1.13.11.12 extracted from

  • Qian, H.; Xia, B.; He, Y.; Lu, Z.; Bie, X.; Zhao, H.; Zhang, C.; Lu, F.
    Expression, purification, and characterization of a novel acidic lipoxygenase from Myxococcus xanthus (2017), Protein Expr. Purif., 138, 13-17 .
    View publication on PubMed
Search for more literature for 1.13.11.12

Application

Application Comment Organism
additional information the enzyme can be used for decolorization purposes in pulp, textile, and wastewater treatment industries Myxococcus xanthus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ complete inhibition at 10 mM Myxococcus xanthus
K+ 38% residual activity at 50 mM Myxococcus xanthus
Zn2+ 53% residual activity at 50 mM Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus
0.262
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 129% activity at 10 mM Myxococcus xanthus
Ca2+ 159% activity at 10 mM Myxococcus xanthus
Fe2+ 159% activity at 10 mM Myxococcus xanthus
Mn2+ 118% activity at 10 mM Myxococcus xanthus
Na+ 158% activity at 50 mM Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q1DBH9
-
-
Myxococcus xanthus DK1622 Q1DBH9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aniline blue + O2 69.7% decolorization after 35 min incubation Myxococcus xanthus ?
-
 ?
aniline blue + O2 69.7% decolorization after 35 min incubation Myxococcus xanthus DK1622 ?
-
 ?
arachidonic acid + O2
-
 Myxococcus xanthus ?
-
 ?
arachidonic acid + O2
-
 Myxococcus xanthus DK1622 ?
-
 ?
linoleic acid + O2 best substrate Myxococcus xanthus (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
linoleic acid + O2 best substrate Myxococcus xanthus DK1622 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Myxococcus xanthus

Synonyms

Synonyms Comment Organism
acidic lipoxygenase
-
 Myxococcus xanthus
LOX
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzyme activity gradually decreases at temperatures above 30°C Myxococcus xanthus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 the relative activity is greater than 60% within the temperature range of 20-40°C Myxococcus xanthus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 the thermal stability is highest at 25°C. Less than 50% of the original activity remains after heat treatment at 50°C for 5 min, and the enzyme is completely inactive after treatment at 60°C for 10 min Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus
66
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Myxococcus xanthus

pH Range

pH Minimum pH Maximum Comment Organism
2.5 3.5 about 85% activity at pH 2.5, 100% activity at pH 3.0, about 85% activity at pH 3.5. The enzyme activity decreases dramatically at pH values greater than 4.0, while catalytic activity is barely detected at pH values of greater than 6.0 Myxococcus xanthus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 the enzyme retains about 95% activity after 10 min at pH 6.0. After 10 min incubation at pH 3.0-5.0 or 7.0-10.0, the enzyme retains about 60-70% of the original activity Myxococcus xanthus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
251.9
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus
277.1
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus