Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 70% inhibition at 20 mM | Glycine max | |
Hg2+ | about 80% inhibition at 20 mM | Glycine max | |
Zn2+ | complete inhibition at 20 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.636 | - |
linoleic acid | at pH 9.5 and 40°C | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 129.79% activity at 20 mM | Glycine max | |
additional information | Mg2+ and Ca2+ have no effect on the enzyme activity | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
black soybean | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiTrap Q column chromatography, DEAE Sepharose column chromatography, and Sephacryl S-100 gel filtration | Glycine max |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
crude enzyme, at pH 9.5 and 40°C | Glycine max |
3 | - |
after 85fold purification, at pH 9.5 and 40°C | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenic acid + O2 | minor substrate | Glycine max | ? | - |
? | |
linoleic acid + O2 | - |
Glycine max | 13(S)-hydroperoxy-9,11-octadecadienoic acid | - |
? | |
additional information | no activity with oleic acid | Glycine max | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 55000 + 1 * 46000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
13S-lipoxygenase | - |
Glycine max |
LOX | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | the enzyme has 51.51% activity at 15°C and 63.97% activity at 55°C | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the enzyme shows no loss of catalytic activity at temperatures up to 40°C after 15 min incubation. When the temperature increases above 40 °C, the enzyme dramatically loses its catalytic activity by 56% at 60°C and is fully deactivated at 70°C | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.28 | - |
linoleic acid | at pH 9.5 and 40°C | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | the enzyme has about 60% activity at pH 8.0 and about 70% activity at pH10.0 | Glycine max |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme is highly stable at pH 6.0-10.0, retaining over 80% of its original catalytic activity after 24 h | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.3 | - |
linoleic acid | at pH 9.5 and 40°C | Glycine max |