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Literature summary for 1.13.11.12 extracted from

  • Patel, D.D.; Patel, R.R.; Thakkar, V.R.
    Purification, characterization and application of lipoxygenase isoenzymes from Lasiodiplodia theobromae (2015), Appl. Biochem. Biotechnol., 175, 513-525 .
    View publication on PubMed

Application

Application Comment Organism
food industry addition of LOX1 and LOX2 altered the elasticity as well as viscosity of dough prepared from bleached wheat flour, LOX improves the dough rheology Lasiodiplodia theobromae

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme LOX1 is not inhibited by jasmonic acid; enzyme LOX2 is not inhibited by jasmonic acid Lasiodiplodia theobromae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Lasiodiplodia theobromae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Lasiodiplodia theobromae
93000
-
gel filtration Lasiodiplodia theobromae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Lasiodiplodia theobromae
-
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 ?
linoleate + O2 Lasiodiplodia theobromae MTCC 3068
-
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 ?

Organism

Organism UniProt Comment Textmining
Lasiodiplodia theobromae
-
-
-
Lasiodiplodia theobromae MTCC 3068
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 203.5fold by gel filtration and anion exchange chromatography Lasiodiplodia theobromae
native enzyme 578fold by gel filtration and anion exchange chromatography Lasiodiplodia theobromae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Lasiodiplodia theobromae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5538
-
purified native enzyme, pH 6.0, 40°C Lasiodiplodia theobromae
15733.3
-
purified native enzyme, pH 6.0, 40°C Lasiodiplodia theobromae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
Lasiodiplodia theobromae 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 ?
linoleate + O2
-
Lasiodiplodia theobromae MTCC 3068 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Lasiodiplodia theobromae
monomer 1 * 93000, SDS-PAGE Lasiodiplodia theobromae

Synonyms

Synonyms Comment Organism
LOX1
-
Lasiodiplodia theobromae
LOX2
-
Lasiodiplodia theobromae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lasiodiplodia theobromae
50
-
-
Lasiodiplodia theobromae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 activity range Lasiodiplodia theobromae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lasiodiplodia theobromae

General Information

General Information Comment Organism
physiological function the lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (HPOD), which is important in food and flavour industries for production of bread and flavouring compounds Lasiodiplodia theobromae