Application | Comment | Organism |
---|---|---|
food industry | addition of LOX1 and LOX2 altered the elasticity as well as viscosity of dough prepared from bleached wheat flour, LOX improves the dough rheology | Lasiodiplodia theobromae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme LOX1 is not inhibited by jasmonic acid; enzyme LOX2 is not inhibited by jasmonic acid | Lasiodiplodia theobromae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Lasiodiplodia theobromae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Lasiodiplodia theobromae |
93000 | - |
gel filtration | Lasiodiplodia theobromae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Lasiodiplodia theobromae | - |
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? | |
linoleate + O2 | Lasiodiplodia theobromae MTCC 3068 | - |
9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lasiodiplodia theobromae | - |
- |
- |
Lasiodiplodia theobromae MTCC 3068 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 203.5fold by gel filtration and anion exchange chromatography | Lasiodiplodia theobromae |
native enzyme 578fold by gel filtration and anion exchange chromatography | Lasiodiplodia theobromae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Lasiodiplodia theobromae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5538 | - |
purified native enzyme, pH 6.0, 40°C | Lasiodiplodia theobromae |
15733.3 | - |
purified native enzyme, pH 6.0, 40°C | Lasiodiplodia theobromae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Lasiodiplodia theobromae | 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? | |
linoleate + O2 | - |
Lasiodiplodia theobromae MTCC 3068 | 9-hydroperoxy octadecadienoic acid + 13-hydroperoxy octadecadienoic acid | 9-hydroperoxy octadecadienoic acid is the product of EC 1.13.11.58 | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Lasiodiplodia theobromae |
monomer | 1 * 93000, SDS-PAGE | Lasiodiplodia theobromae |
Synonyms | Comment | Organism |
---|---|---|
LOX1 | - |
Lasiodiplodia theobromae |
LOX2 | - |
Lasiodiplodia theobromae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lasiodiplodia theobromae |
50 | - |
- |
Lasiodiplodia theobromae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | activity range | Lasiodiplodia theobromae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Lasiodiplodia theobromae |
General Information | Comment | Organism |
---|---|---|
physiological function | the lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (HPOD), which is important in food and flavour industries for production of bread and flavouring compounds | Lasiodiplodia theobromae |