Literature summary for 1.13.11.12 extracted from

Palmieri-Thiers, C.; Canaan, S.; Brunini, V.; Lorenzi, V.; Tomi, F.; Desseyn, J.L.; Garscha, U.; Oliw, E.H.; Berti, L.; Maury, J.
A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening (2009), Biochim. Biophys. Acta, 1791, 339-346.

Search for more literature for 1.13.11.12

Cloned(Commentary)

Cloned (Comment)	Organism
vector pQE30-LOX expressed in Escherichia coli M15 cells	Olea europaea

Inhibitors

Inhibitors	Comment	Organism	Structure
nordihydroguaiaretic acid	is a noncompetitive inhibitor	Olea europaea
propyl gallate	is a competitive inhibitor	Olea europaea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1749	-	linoleic acid	pH 6.0, 25°C	Olea europaea
0.3437	-	linolenic acid	pH 6.0, 25°C	Olea europaea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
98000	-	x * 98000, SDS-PAGE and immunoblot	Olea europaea
98400	-	x * 98400, calculated from sequence	Olea europaea

Organism

Organism	UniProt	Comment	Textmining
Olea europaea	B6D1W5	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-affinity chromatography and gel filtration, 26fold	Olea europaea

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	is mainly expressed at late developmental stages, two Lox genes expressed in black olives. Increase of the LOX activity at the end of the green stage and the turning stage of the olives, and the maximum is reached at the black stage	Olea europaea	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
23.4	-	26fold purified enzyme, with linoleic acid as substrate	Olea europaea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
linoleic acid + O2	-	Olea europaea	(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid	the recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxy octadecadienoic acid in a ratio 2:1	?
linolenic acid + O2	-	Olea europaea	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 98000, SDS-PAGE and immunoblot	Olea europaea
?	x * 98400, calculated from sequence	Olea europaea

Synonyms

Synonyms	Comment	Organism
9/13-LOX	-	Olea europaea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
47.78	-	linolenic acid	pH 6.0, 25°C	Olea europaea
48.16	-	linoleic acid	pH 6.0, 25°C	Olea europaea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Olea europaea

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00188	-	propyl gallate	pH 6.0, 25°C	Olea europaea
0.00409	-	nordihydroguaiaretic acid	pH 6.0, 25°C	Olea europaea

pI Value

Organism	Comment	pI Value Maximum	pI Value
Olea europaea	calculated from sequence	-	5.95

General Information

General Information	Comment	Organism
physiological function	9/13-LOX is associated with the ripening and senescence processes	Olea europaea

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
140	-	linolenic acid	-	Olea europaea
280	-	linoleic acid	-	Olea europaea

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)