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Literature summary for 1.13.11.11 extracted from

  • Zhang, S.; Guo, L.; Yang, D.; Xing, Z.; Li, W.; Kuang, C.; Yang, Q.
    Evaluation and comparison of the commonly used bioassays of human indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) (2020), Bioorg. Chem., 104, 104348 .
    View publication on PubMed
Search for more literature for 1.13.11.11

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzymatic and cellular assays are influenced by many factors including detection product, reaction time and culture medium, which may cause the inconsistency of enzymatic and cellular inhibitory activities of test compounds. The TDO IC50 values of all the test compounds derived from NFK assay are 2-3 times higher than that of Kyn adduct assay Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.177
-
 L-tryptophan pH 7.0, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + O2 Homo sapiens
-
 N-formyl-L-kynurenine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P48775
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-172 cell
-
 Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?

Synonyms

Synonyms Comment Organism
TDO
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.61
-
 L-tryptophan pH 7.0, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 the enzymatic and cellular assays are influenced by many factors including detection product, reaction time and culture medium, which may cause the inconsistency of enzymatic and cellular inhibitory activities of test compounds. The IC50 values of all the test compounds derived from NFK assay are 2-3 times higher than that of Kyn adduct assay Homo sapiens additional information

General Information

General Information Comment Organism
physiological function tryptophan 2,3-dioxygenase catalyzes the oxidation of the essential amino acid tryptophan to N-formylkynurenine which is rapidly converted to a series of biological active metabolites including kynurenine via the kynurenine pathway Homo sapiens