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Literature summary for 1.13.11.1 extracted from
- Purification and characterization of catechol 1,2-dioxygenase from Acinetobacter sp. Y64 strain and Escherichia coli transformants (2015), Protein J., 34, 421-433 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acinetobacter sp. Y64 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 58% residual activity at 0.5 mM | Acinetobacter sp. Y64 |  |
| EDTA | 50% residual activity at 0.5 mM | Acinetobacter sp. Y64 | |
| additional information | not inhibited by Hg2+ | Acinetobacter sp. Y64 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01753 | - |
catechol | at pH 7.0 and 30°C | Acinetobacter sp. Y64 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 144% activity at 0.5 mM | Acinetobacter sp. Y64 | |
| Fe3+ | 164% activity at 0.5 mM | Acinetobacter sp. Y64 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Acinetobacter sp. Y64 | 100% activity | cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter sp. Y64 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Hitrap QFF column chromatography | Acinetobacter sp. Y64 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.42 | - |
crude enzyme, at pH 7.0 and 30°C | Acinetobacter sp. Y64 |
| 15.13 | - |
afer 10.6fold purification, at pH 7.0 and 30°C | Acinetobacter sp. Y64 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2,4-benzenetriol + O2 | 52% activity compared to catechol | Acinetobacter sp. Y64 | ? | - |
? | |
| 3-methylcatechol + O2 | 2% activity compared to catechol | Acinetobacter sp. Y64 | ? | - |
? | |
| 4-methylcatechol + O2 | 61% activity compared to catechol | Acinetobacter sp. Y64 | ? | - |
? | |
| 4-nitrocatechol + O2 | 80% activity compared to catechol | Acinetobacter sp. Y64 | ? | - |
? | |
| catechol + O2 | 100% activity | Acinetobacter sp. Y64 | cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Acinetobacter sp. Y64 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C1,2O | - |
Acinetobacter sp. Y64 |
| CatA | - |
Acinetobacter sp. Y64 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Acinetobacter sp. Y64 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | the enzyme is stable at the optimal temperature at 37°C for 5 h and loses 10% of its activity after 2 h. As the temperature increases, the enzyme activity declines (37% of activity remains at 45°C under the assay conditions, while the enzyme loses approximately 90% activity in 1 h at 50 and 55°C, respectively) | Acinetobacter sp. Y64 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Acinetobacter sp. Y64 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
at pH 7.0 and 30°C | Acinetobacter sp. Y64 | EDTA | |
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