Cloned (Comment) | Organism |
---|---|
gene catA, DNA and amino acid sequence determination and analysis | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Acinetobacter sp. | - |
cis,cis-muconate | - |
? | |
catechol + O2 | Acinetobacter sp. DS002 | - |
cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
gene catA | - |
Acinetobacter sp. DS002 | - |
gene catA | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity from cell supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, folloed by gel filtration | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene-1,2,4-triol + O2 | 16% of the activity with catechol | Acinetobacter sp. | ? | - |
? | |
benzene-1,2,4-triol + O2 | 16% of the activity with catechol | Acinetobacter sp. DS002 | ? | - |
? | |
catechol + O2 | - |
Acinetobacter sp. | cis,cis-muconate | - |
? | |
catechol + O2 | best substrate | Acinetobacter sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Acinetobacter sp. DS002 | cis,cis-muconate | - |
? | |
catechol + O2 | best substrate | Acinetobacter sp. DS002 | cis,cis-muconate | - |
? | |
additional information | poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol | Acinetobacter sp. | ? | - |
? | |
additional information | poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol | Acinetobacter sp. DS002 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 66000, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
C12O | - |
Acinetobacter sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | catechol 1,2-dioxygenase is the key enzyme in ortho pathway of catechol degradation | Acinetobacter sp. |