Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Acinetobacter radioresistens |
Protein Variants | Comment | Organism |
---|---|---|
A72D | site-directed mutagenesis, the mutant shows a low level of iron incorporation compared to the wild-type enzyme and altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
A72G | site-directed mutagenesis, the mutant shows altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
A72N | site-directed mutagenesis, the mutant shows a disturbed iron binding and altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
A72P | site-directed mutagenesis, the mutant shows altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
A72S | site-directed mutagenesis, the mutant shows altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
L69A | site-directed mutagenesis, the mutant shows altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
L69G/A72G | site-directed mutagenesis, the mutant shows altered thermal stability values, pH and temperature dependence | Acinetobacter radioresistens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | bound at the active site | Acinetobacter radioresistens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Acinetobacter radioresistens | - |
cis,cis-muconate | - |
? | |
catechol + O2 | Acinetobacter radioresistens S13 | - |
cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter radioresistens | - |
- |
- |
Acinetobacter radioresistens S13 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Acinetobacter radioresistens | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Acinetobacter radioresistens S13 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,2-CTD | - |
Acinetobacter radioresistens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 27 | mutant L69G/A72G | Acinetobacter radioresistens |
27 | - |
mutant L69A | Acinetobacter radioresistens |
30 | - |
wild-type enzyme and mutants Aa72G and A72P | Acinetobacter radioresistens |
37 | - |
mutant A72S | Acinetobacter radioresistens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.04 | - |
catechol | pH 8.0, 35°C, mutant A72G/L69G | Acinetobacter radioresistens | |
4.81 | - |
catechol | pH 8.0, 35°C, mutant L69A | Acinetobacter radioresistens | |
13.56 | - |
catechol | pH 8.0, 35°C, mutant A72G | Acinetobacter radioresistens | |
14.72 | - |
catechol | pH 8.0, 35°C, mutant A72P | Acinetobacter radioresistens | |
15.48 | - |
catechol | pH 8.0, 35°C, mutant A72S | Acinetobacter radioresistens | |
30.03 | - |
catechol | pH 8.0, 35°C, wild-type enzyme | Acinetobacter radioresistens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
mutant L69G/A72G | Acinetobacter radioresistens |
8 | 8.5 | wild-type enzyme | Acinetobacter radioresistens |
8 | 9 | mutants L69A and A72S | Acinetobacter radioresistens |
8.5 | 9 | mutant A72P | Acinetobacter radioresistens |
9 | - |
mutant A72G | Acinetobacter radioresistens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a non-heme Fe dioxygenase | Acinetobacter radioresistens |
additional information | structure-function relationships of wild-type and mutant enzymes, overview | Acinetobacter radioresistens |