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Literature summary for 1.13.11.1 extracted from

  • Caglio, R.; Valetti, F.; Caposio, P.; Gribaudo, G.; Pessione, E.; Giunta, C.
    Fine-tuning of catalytic properties of catechol 1,2-dioxygenase by active site tailoring (2009), ChemBioChem, 10, 1015-1024.
    View publication on PubMed
Search for more literature for 1.13.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Acinetobacter radioresistens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Acinetobacter baylyi ADP1

Protein Variants

Protein Variants Comment Organism
A72D enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
A72G enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
A72K completely inactive Acinetobacter radioresistens
A72N enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
A72S enhanced substrate specificity towards chlorinated substrates Acinetobacter radioresistens
L69A specificity preference towards 4-chlorocatechol Acinetobacter radioresistens
L69G/A72G heavily destabilised Acinetobacter radioresistens
additional information catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template Acinetobacter radioresistens
additional information used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13 Acinetobacter baylyi ADP1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000101
-
 4-Chlorocatechol mutant L69A Acinetobacter radioresistens
0.00198
-
 4-Chlorocatechol wild-type enzyme Acinetobacter radioresistens
0.00204
-
 catechol wild-type enzyme Acinetobacter radioresistens
0.00225
-
 3-methylcatechol wild-type enzyme Acinetobacter radioresistens
0.00235
-
 catechol mutant A72N Acinetobacter radioresistens
0.00242
-
 4-Chlorocatechol mutant L69G/A72G Acinetobacter radioresistens
0.00284
-
 4-methylcatechol mutant L69G/A72G Acinetobacter radioresistens
0.00576
-
 catechol mutant L69A Acinetobacter radioresistens
0.00595
-
 3-methylcatechol mutant L69A Acinetobacter radioresistens
0.00613
-
 4-Chlorocatechol mutant A72G Acinetobacter radioresistens
0.00654
-
 4-Chlorocatechol mutant A72S Acinetobacter radioresistens
0.0093
-
 catechol mutant A72G Acinetobacter radioresistens
0.01
-
 4-methylcatechol mutant A72G Acinetobacter radioresistens
0.01
-
 catechol mutant A72S Acinetobacter radioresistens
0.0128
-
 4-methylcatechol mutant L69A Acinetobacter radioresistens
0.0428
-
 catechol mutant L69G/A72G Acinetobacter radioresistens
0.0501
-
 4-Chlorocatechol mutant A72N Acinetobacter radioresistens
0.053
-
 4-methylcatechol wild-type enzyme Acinetobacter radioresistens
0.0691
-
 catechol mutant A72D Acinetobacter radioresistens
0.0936
-
 4-methylcatechol mutant A72D Acinetobacter radioresistens
0.2081
-
 4-Chlorocatechol mutant A72D Acinetobacter radioresistens
0.213
-
 4-methylcatechol mutant A72S Acinetobacter radioresistens
0.435
-
 4-methylcatechol mutant A72N Acinetobacter radioresistens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ Fe(III)-dependent Acinetobacter radioresistens

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi ADP1 P07773 used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13
-
Acinetobacter radioresistens Q9F103 catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template
-
Acinetobacter radioresistens S13 Q9F103 catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter radioresistens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
3-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
4,5-dichlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
4,5-dichlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
4-chlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
4-chlorocatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
4-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
4-methylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
4-tert-butylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens ?
-
 ?
4-tert-butylcatechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens S13 ?
-
 ?
catechol + O2 assay at pH 8.0, 30°C Acinetobacter radioresistens cis-cis muconate
-
 ?

Synonyms

Synonyms Comment Organism
catechol 1,2-dioxygenase
-
 Acinetobacter radioresistens
catechol 1,2-dioxygenase
-
 Acinetobacter baylyi ADP1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32.7 51.8 differences between wild-type and mutant enzymes Acinetobacter radioresistens

pH Range

pH Minimum pH Maximum Comment Organism
6.89 7.49 differs between wild-type and mutant enzymes Acinetobacter radioresistens