Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Acinetobacter radioresistens |
Crystallization (Comment) | Organism |
---|---|
- |
Acinetobacter baylyi ADP1 |
Protein Variants | Comment | Organism |
---|---|---|
A72D | enhanced substrate specificity towards chlorinated substrates | Acinetobacter radioresistens |
A72G | enhanced substrate specificity towards chlorinated substrates | Acinetobacter radioresistens |
A72K | completely inactive | Acinetobacter radioresistens |
A72N | enhanced substrate specificity towards chlorinated substrates | Acinetobacter radioresistens |
A72S | enhanced substrate specificity towards chlorinated substrates | Acinetobacter radioresistens |
L69A | specificity preference towards 4-chlorocatechol | Acinetobacter radioresistens |
L69G/A72G | heavily destabilised | Acinetobacter radioresistens |
additional information | catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template | Acinetobacter radioresistens |
additional information | used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13 | Acinetobacter baylyi ADP1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000101 | - |
4-Chlorocatechol | mutant L69A | Acinetobacter radioresistens | |
0.00198 | - |
4-Chlorocatechol | wild-type enzyme | Acinetobacter radioresistens | |
0.00204 | - |
catechol | wild-type enzyme | Acinetobacter radioresistens | |
0.00225 | - |
3-methylcatechol | wild-type enzyme | Acinetobacter radioresistens | |
0.00235 | - |
catechol | mutant A72N | Acinetobacter radioresistens | |
0.00242 | - |
4-Chlorocatechol | mutant L69G/A72G | Acinetobacter radioresistens | |
0.00284 | - |
4-methylcatechol | mutant L69G/A72G | Acinetobacter radioresistens | |
0.00576 | - |
catechol | mutant L69A | Acinetobacter radioresistens | |
0.00595 | - |
3-methylcatechol | mutant L69A | Acinetobacter radioresistens | |
0.00613 | - |
4-Chlorocatechol | mutant A72G | Acinetobacter radioresistens | |
0.00654 | - |
4-Chlorocatechol | mutant A72S | Acinetobacter radioresistens | |
0.0093 | - |
catechol | mutant A72G | Acinetobacter radioresistens | |
0.01 | - |
4-methylcatechol | mutant A72G | Acinetobacter radioresistens | |
0.01 | - |
catechol | mutant A72S | Acinetobacter radioresistens | |
0.0128 | - |
4-methylcatechol | mutant L69A | Acinetobacter radioresistens | |
0.0428 | - |
catechol | mutant L69G/A72G | Acinetobacter radioresistens | |
0.0501 | - |
4-Chlorocatechol | mutant A72N | Acinetobacter radioresistens | |
0.053 | - |
4-methylcatechol | wild-type enzyme | Acinetobacter radioresistens | |
0.0691 | - |
catechol | mutant A72D | Acinetobacter radioresistens | |
0.0936 | - |
4-methylcatechol | mutant A72D | Acinetobacter radioresistens | |
0.2081 | - |
4-Chlorocatechol | mutant A72D | Acinetobacter radioresistens | |
0.213 | - |
4-methylcatechol | mutant A72S | Acinetobacter radioresistens | |
0.435 | - |
4-methylcatechol | mutant A72N | Acinetobacter radioresistens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | Fe(III)-dependent | Acinetobacter radioresistens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi ADP1 | P07773 | used as a template for catechol 1,2-dioxygenase from Acinetobacter radioresistens strain S13 | - |
Acinetobacter radioresistens | Q9F103 | catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template | - |
Acinetobacter radioresistens S13 | Q9F103 | catechol 1,2-dioxygenase from Acinetobacter ADP1 is used as a template | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter radioresistens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | ? | - |
? | |
3-methylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens S13 | ? | - |
? | |
4,5-dichlorocatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | ? | - |
? | |
4,5-dichlorocatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens S13 | ? | - |
? | |
4-chlorocatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | ? | - |
? | |
4-chlorocatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens S13 | ? | - |
? | |
4-methylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | ? | - |
? | |
4-methylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens S13 | ? | - |
? | |
4-tert-butylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | ? | - |
? | |
4-tert-butylcatechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens S13 | ? | - |
? | |
catechol + O2 | assay at pH 8.0, 30°C | Acinetobacter radioresistens | cis-cis muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
catechol 1,2-dioxygenase | - |
Acinetobacter radioresistens |
catechol 1,2-dioxygenase | - |
Acinetobacter baylyi ADP1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
32.7 | 51.8 | differences between wild-type and mutant enzymes | Acinetobacter radioresistens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.89 | 7.49 | differs between wild-type and mutant enzymes | Acinetobacter radioresistens |