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Literature summary for 1.13.11.1 extracted from

  • Nakai, C.; Horiike, K.; Kuramitsu, S.; Kagamiyama, H.; Nozaki, M.
    Three isoenzymes of catechol 1,2-dioxygenase (pyrocatechase) alphaalpha, alphabeta, and betabeta from Pseudomonas arvilla C-1 (1980), J. Biol. Chem., 265, 660-665.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
catechol isoenzymes 1 and 3 Pseudomonas putida
0.002
-
catechol isoenzyme 2 Pseudomonas putida
0.096
-
oxygen isoenzymes 1 and 2 Pseudomonas putida
0.098
-
oxygen isoenzyme 3 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1 gatom per mol enzyme protein Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, isoenzyme 3, SDS-PAGE Pseudomonas putida
30000
-
1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
32000
-
2 * 32000, isoenzyme 1, SDS-PAGE Pseudomonas putida
32000
-
1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
59000
-
isoenzyme 3, gel filtration Pseudomonas putida
64000
-
isoenzyme 2, gel filtration Pseudomonas putida
66900
-
isoenzyme 1, gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
C-1
-
Pseudomonas putida C-1
-
C-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 poor substrate Pseudomonas putida (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
3-methylcatechol + O2 poor substrate Pseudomonas putida C-1 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2
-
Pseudomonas putida (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
4-methylcatechol + O2
-
Pseudomonas putida C-1 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
catechol + O2
-
Pseudomonas putida cis,cis-muconate
-
?
catechol + O2
-
Pseudomonas putida C-1 cis,cis-muconate
-
?
additional information both intradiol and extradiol cleavage activities toward 3-substituted catechols Pseudomonas putida ?
-
?
additional information both intradiol and extradiol cleavage activities toward 3-substituted catechols Pseudomonas putida C-1 ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
homodimer 2 * 32000, isoenzyme 1, SDS-PAGE Pseudomonas putida
homodimer 2 * 30000, isoenzyme 3, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
catechol isoenzyme 3 Pseudomonas putida
49
-
catechol isoenzyme 2 Pseudomonas putida
54
-
catechol isoenzyme 1 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
isoenzymes 1,2 and 3 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 isoenzymes 1,2 and 3 Pseudomonas putida