KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
catechol | isoenzymes 1 and 3 | Pseudomonas putida | |
0.002 | - |
catechol | isoenzyme 2 | Pseudomonas putida | |
0.096 | - |
oxygen | isoenzymes 1 and 2 | Pseudomonas putida | |
0.098 | - |
oxygen | isoenzyme 3 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1 gatom per mol enzyme protein | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, isoenzyme 3, SDS-PAGE | Pseudomonas putida |
30000 | - |
1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE | Pseudomonas putida |
32000 | - |
2 * 32000, isoenzyme 1, SDS-PAGE | Pseudomonas putida |
32000 | - |
1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE | Pseudomonas putida |
59000 | - |
isoenzyme 3, gel filtration | Pseudomonas putida |
64000 | - |
isoenzyme 2, gel filtration | Pseudomonas putida |
66900 | - |
isoenzyme 1, gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
C-1 | - |
Pseudomonas putida C-1 | - |
C-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | poor substrate | Pseudomonas putida | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
3-methylcatechol + O2 | poor substrate | Pseudomonas putida C-1 | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas putida | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas putida C-1 | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | - |
Pseudomonas putida | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Pseudomonas putida C-1 | cis,cis-muconate | - |
? | |
additional information | both intradiol and extradiol cleavage activities toward 3-substituted catechols | Pseudomonas putida | ? | - |
? | |
additional information | both intradiol and extradiol cleavage activities toward 3-substituted catechols | Pseudomonas putida C-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE | Pseudomonas putida |
homodimer | 2 * 32000, isoenzyme 1, SDS-PAGE | Pseudomonas putida |
homodimer | 2 * 30000, isoenzyme 3, SDS-PAGE | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
catechol | isoenzyme 3 | Pseudomonas putida | |
49 | - |
catechol | isoenzyme 2 | Pseudomonas putida | |
54 | - |
catechol | isoenzyme 1 | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
isoenzymes 1,2 and 3 | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | isoenzymes 1,2 and 3 | Pseudomonas putida |