Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 64% inactivation, 5 mM, 30 min, 24°C | Pseudomonas putida | |
Tiron | 48% inactivation, 5 mM, 30 min, 24°C | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
catechol | - |
Pseudomonas putida | |
0.06 | - |
oxygen | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1.1 gatom per mol enzyme protein based on a molecular weight of 65000 Da | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Pseudomonas putida |
64000 | 66000 | gel filtration, sedimentation equilibrium analysis | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
mt-2, ATCC 23973, TN2100 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, pH 8.5, stable over a period of several months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | poor substrate | Pseudomonas putida | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4-chlorocatechol + O2 | poor substrate | Pseudomonas putida | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas putida | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | - |
Pseudomonas putida | cis,cis-muconate | - |
? | |
additional information | no substrates: 4-nitrocatechol, protocatechuic acid, ratios of intradiol and extradiol cleavage activities for 3-methylcatechol and 3-methoxycatechol 22 : 4.3 | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Pseudomonas putida |