Any feedback?
Literature summary for 1.13.11.1 extracted from
- Studies on oxygenases: pyrocatechase (1957), J. Biol. Chem., 229, 905-920.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | inhibition can almost completely be reversed by addition of glutathione | Pseudomonas fluorescens |  |
| Cu2+ | - |
Pseudomonas fluorescens | |
| Hg2+ | inhibition can almost completely reserved by addition of glutathione | Pseudomonas fluorescens | |
| o-benzoquinone | - |
Pseudomonas fluorescens | |
| p-chloromercuribenzoate | inhibition can almost completely be reversed by addition of glutathione | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 2 atoms per molecule of enzyme protein | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
- |
Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
ATCC 11250 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, pH 9.0, 5-10% loss of activity after several months | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | - |
Pseudomonas fluorescens | cis,cis-muconate | - |
? | |
| additional information | no substrates: adrenolutine, p-aminocatechol, protocatechuic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, trans-5,6-dihydroxycyclohexadiene, pyrogallol, dopa, homogentisic acid, gentisic acid | Pseudomonas fluorescens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.7 | - |
catechol | - |
Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | phosphate buffer | Pseudomonas fluorescens |
| 7 | 10 | phosphate buffer, addition of glutathione | Pseudomonas fluorescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
