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Literature summary for 1.12.99.6 extracted from

  • Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.
    High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form (2015), Protein Expr. Purif., 107, 90-94 .
    View publication on PubMed
Search for more literature for 1.12.99.6

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pyrococcus furiosus 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NADPH Pyrococcus furiosus
-
 NADP + H+
-
 ?
H2 + NADPH Pyrococcus furiosus MW0430
-
 NADP + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus MW0430
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NADPH
-
 Pyrococcus furiosus NADP + H+
-
 ?
H2 + NADPH
-
 Pyrococcus furiosus MW0430 NADP + H+
-
 ?

Subunits

Subunits Comment Organism
heterotetramer
-
 Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
SHI
-
 Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pyrococcus furiosus
[2Fe-2S]-center
-
 Pyrococcus furiosus
[4Fe-4S]-center
-
 Pyrococcus furiosus