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Literature summary for 1.12.99.6 extracted from
- Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe] hydrogenase of Ralstonia eutropha H16 (2009), J. Biol. Chem., 284, 16264-16276.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in a non-tagged version using plasmid pLO6, harboring the complete MBH operon, in Ralstonia eutropha strain HF631 | Cupriavidus necator H16 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | is oxygen-tolerant | Cupriavidus necator H16 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | the hydrogenase dimer is electronically connected to a membrane-integral cytochrome b | Cupriavidus necator H16 | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CN- | the catalytic center is equipped with 1.8 CN- per protein molecule | Cupriavidus necator H16 |  |
| CO | the catalytic center is equipped with one CO | Cupriavidus necator H16 | |
| Fe | is composed of a small subunit, capable in coordinating one [3Fe4S] and two [4Fe4S] clusters | Cupriavidus necator H16 | |
| Ni2+ | is composed of a large subunit, harboring the [NiFe] active site | Cupriavidus necator H16 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator H16 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography, ca. 99% pure | Cupriavidus necator H16 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized acceptor | almost 100% of the membrane-attached MBH is reversibly redox-active | Cupriavidus necator H16 | H+ + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | detachment of the enzyme from the membrane | Cupriavidus necator H16 |
| trimer | in its native environment | Cupriavidus necator H16 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mbh | - |
Cupriavidus necator H16 |
| [NiFe] hydrogenase | - |
Cupriavidus necator H16 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
solubilization of the imer is associated with a downshift of the optimum pH for H2-oxidizing activity | Cupriavidus necator H16 |
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Release 2023.1 (January 2023)
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