Protein Variants | Comment | Organism |
---|---|---|
additional information | the uptake activity in mutant HD705/pBS(Kan), which is defective in hydrogenase 3, is 4.4fold lower than that in wild-type enzyme, the hydrogen uptake activity in the hydrogenase 1 and 2 double mutant (hyaB hybC) is reduced 2.7fold by addition of the hycE mutation (hyaB hybC hycE) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CN- | contains two CN- molecules at the active site | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
hydrogenase 3, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains BW25113 and MC4100 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
hydrogen uptake specific activity of the hydrogenase 3 lacking mutant enzyme HD705/pBS(Kan), using methyl viologen as a substrate | Escherichia coli |
0.035 | - |
hydrogen uptake specific activity of the wild type enzyme, using methyl viologen as a substrate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | - |
Escherichia coli | acceptor-H2 | - |
? | |
H2 + oxidized methyl viologen | - |
Escherichia coli | H+ + reduced methyl viologen | - |
r |
Synonyms | Comment | Organism |
---|---|---|
HycE | hydrogenase 3 | Escherichia coli |
hydrogen uptake hydrogenase | - |
Escherichia coli |
hydrogenase 1 | hydrogen uptake activity only | Escherichia coli |
hydrogenase 2 | hydrogen uptake activity only | Escherichia coli |
hydrogenase 3 | hydrogen uptake and synthesis activity | Escherichia coli |
[NiFe]-hydrogenase | - |
Escherichia coli |