Cloned (Comment) | Organism |
---|---|
expression of Strep-tagged enzyme | Wolinella succinogenes |
Protein Variants | Comment | Organism |
---|---|---|
A204F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
D100N | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
D88N | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
E94Q | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
G125L | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
H186A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
H200A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
H25A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
H67A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
M203I | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
N128D | 5% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
P129A | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
Q131L | 2% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
Y114F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
Y127A | 9% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
Y127F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
Y127H | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
Y202F | almost no activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound, enzyme is oriented to the periplasmic space | Wolinella succinogenes | 16020 | - |
periplasm | - |
Wolinella succinogenes | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | FeNi-hydrogenase | Wolinella succinogenes | |
Ni | FeNi-hydrogenase | Wolinella succinogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wolinella succinogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Wolinella succinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + 2,3-dimethyl-1,4-naphthoquinone | - |
Wolinella succinogenes | 2,3-dimethyl-naphthoquinol | - |
? | |
H2 + oxidized benzyl viologen | - |
Wolinella succinogenes | reduced benzyl viologen + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | subunits HydA, HydB and HydC | Wolinella succinogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | involved in electron transport | Wolinella succinogenes |