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Literature summary for 1.12.99.6 extracted from
- Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12 (1986), Eur. J. Biochem., 156, 265-275.
General Stability
| General Stability | Organism |
|---|---|
| 90% loss of activity after 8 days in the presence of oxygen, increasing ionic strength causes 10-40% reversible inhibition | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 95% inhibition at 0.1 mM | Escherichia coli |  |
| N-bromosuccinimide | complete inactivation at 10 mM, 20% inactivation at 0.1 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
H2 | - |
Escherichia coli | |
| 0.0045 | - |
benzyl viologen | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 12.2 mol per mol enzyme, iron-sulfur protein | Escherichia coli | |
| Sulfide | 9.1 mol per mol enzyme, iron-sulfur protein | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
| 31000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
| 64000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
| 200000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.6 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | benzyl viologen, methylene blue and methylviologen can act as electron acceptors | Escherichia coli | H+ + reduced acceptor | - |
? | |
| H2 + oxidized benzyl viologen | - |
Escherichia coli | H+ + reduced benzyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | enzyme is still 60% active after 4 h at pH 4 or pH 10 | Escherichia coli |
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