Literature summary for 1.12.98.4 extracted from

Laska, S.; Lottspeich, F.; Kletzin, A.
Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens (2003), Microbiology, 149, 2357-2371.

Search for more literature for 1.12.98.4

Cloned(Commentary)

Cloned (Comment)	Organism
the SR gene cluster encompasses five ORFs with a total length of 7389 bp. The sreA gene encodes the 110000 Da subunit of SR. sreB encodes an FeS protein, sreC encodes a hydrophobic protein with 10 predicted transmembrane helices. The sreD gene encodes a putative hydrophilic FeS protein with 26 cysteine residues and of unknown cluster composition. The sreE gene encodes a protein with similarity to reductase assembly proteins	Acidianus ambivalens

Cloned (Comment)

Organism

the SR gene cluster encompasses five ORFs with a total length of 7389 bp. The sreA gene encodes the 110000 Da subunit of SR. sreB encodes an FeS protein, sreC encodes a hydrophobic protein with 10 predicted transmembrane helices. The sreD gene encodes a putative hydrophilic FeS protein with 26 cysteine residues and of unknown cluster composition. The sreE gene encodes a protein with similarity to reductase assembly proteins

Acidianus ambivalens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	bound to	Acidianus ambivalens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Acidianus ambivalens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acidianus ambivalens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sulfur + H2	in solubilized membrane fraction no activity is detected without the addition of electron carriers: 2,3-dimethylnaphthoquinone, plastoquinone or horse heart cytochrome c	Acidianus ambivalens	H2S	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate	Acidianus ambivalens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate	Acidianus ambivalens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	addition increases activity	Acidianus ambivalens