BRENDA - Enzyme Database
show all sequences of 1.12.98.4

Sulfur reductases from spirilloid mesophilic sulfur-reducing eubacteria

Fauque, G.D.; Klimmek, O.; Kroger, A.; Methods Enzymol. 243, 367-383 (1994)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
GSH
-
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Wolinella succinogenes
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Sulfurospirillum deleyianum
16020
-
membrane
oriented toward the periplasmic side
Wolinella succinogenes
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
iron-sulfur (non-heme) protein
Sulfurospirillum deleyianum
Fe
0.12 mM nonheme iron per g protein, Psr B (Fe-S)-centers; iron-sulfur (non-heme) protein
Wolinella succinogenes
Mo
enzym contains molybdenum 0.006 mmol/g protein bound to a pterin dinucleotide
Wolinella succinogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur
Wolinella succinogenes
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
H2S
-
Wolinella succinogenes
?
sulfur
Sulfurospirillum deleyianum
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
H2S
-
Sulfurospirillum deleyianum
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Triton X-100
-
Sulfurospirillum deleyianum
Triton X-100
-
Wolinella succinogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfurospirillum deleyianum
-
-
-
Wolinella succinogenes
-
DSM 1740; enzyme formed during growth at the expense of sulfur reduction by formate
-
Purification (Commentary)
Commentary
Organism
-
Sulfurospirillum deleyianum
isolation and incorporation into liposomes
Wolinella succinogenes
Reaction
Reaction
Commentary
Organism
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1
sulfur can be reduced with hydrogen as donor in the presence of hydrogenase
Sulfurospirillum deleyianum
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1
polysulfide appears to be the soluble intermediate in the sulfur reduction; sulfur can be reduced with hydrogen as donor in the presence of hydrogenase
Wolinella succinogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.27
-
crude extract
Sulfurospirillum deleyianum
732
-
-
Wolinella succinogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
sulfur can be reduced by photochemical reduction in the presence of phenosafranin
437774
Sulfurospirillum deleyianum
?
-
-
-
-
organic trisulfides + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
-
-
?
polysulfide + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437774
Sulfurospirillum deleyianum
?
polysulfide + electron donor
polysulfide appears to be the soluble intermediate in the sulfur reduction, electron-transport-coupled phosphorylation
437774
Wolinella succinogenes
H2S + oxidized electron donor
-
437774
Wolinella succinogenes
?
sulfur
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
437774
Wolinella succinogenes
H2S
-
437774
Wolinella succinogenes
?
sulfur
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
437774
Sulfurospirillum deleyianum
H2S
-
437774
Sulfurospirillum deleyianum
?
sulfur + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437774
Sulfurospirillum deleyianum
?
sulfur + electron donor
electron donor: molecular hydrogen
437774
Wolinella succinogenes
H2S + oxidized electron donor
-
437774
Wolinella succinogenes
?
Subunits
Subunits
Commentary
Organism
More
SDS-PAGE, Psr A: 85000 Da and Psr B: 23000 Da, amount to approximately 70% of the total protein and are present at nearly equimolar quantities, Psr C not identified
Wolinella succinogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
8.9
-
Sulfurospirillum deleyianum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
GSH
-
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Wolinella succinogenes
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Sulfurospirillum deleyianum
16020
-
membrane
oriented toward the periplasmic side
Wolinella succinogenes
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
iron-sulfur (non-heme) protein
Sulfurospirillum deleyianum
Fe
0.12 mM nonheme iron per g protein, Psr B (Fe-S)-centers; iron-sulfur (non-heme) protein
Wolinella succinogenes
Mo
enzym contains molybdenum 0.006 mmol/g protein bound to a pterin dinucleotide
Wolinella succinogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur
Wolinella succinogenes
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
H2S
-
Wolinella succinogenes
?
sulfur
Sulfurospirillum deleyianum
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
H2S
-
Sulfurospirillum deleyianum
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Triton X-100
-
Sulfurospirillum deleyianum
Triton X-100
-
Wolinella succinogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sulfurospirillum deleyianum
isolation and incorporation into liposomes
Wolinella succinogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.27
-
crude extract
Sulfurospirillum deleyianum
732
-
-
Wolinella succinogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
sulfur can be reduced by photochemical reduction in the presence of phenosafranin
437774
Sulfurospirillum deleyianum
?
-
-
-
-
organic trisulfides + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
-
-
?
polysulfide + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437774
Sulfurospirillum deleyianum
?
polysulfide + electron donor
polysulfide appears to be the soluble intermediate in the sulfur reduction, electron-transport-coupled phosphorylation
437774
Wolinella succinogenes
H2S + oxidized electron donor
-
437774
Wolinella succinogenes
?
sulfur
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
437774
Wolinella succinogenes
H2S
-
437774
Wolinella succinogenes
?
sulfur
reduction is coupled to the phosphorylation of ADP with phosphate in the catabolism of some anaerobic microorganisms
437774
Sulfurospirillum deleyianum
H2S
-
437774
Sulfurospirillum deleyianum
?
sulfur + electron donor
-
437774
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437774
Sulfurospirillum deleyianum
?
sulfur + electron donor
electron donor: molecular hydrogen
437774
Wolinella succinogenes
H2S + oxidized electron donor
-
437774
Wolinella succinogenes
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
SDS-PAGE, Psr A: 85000 Da and Psr B: 23000 Da, amount to approximately 70% of the total protein and are present at nearly equimolar quantities, Psr C not identified
Wolinella succinogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
8.9
-
Sulfurospirillum deleyianum
Other publictions for EC 1.12.98.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716255
Yang
SurR regulates hydrogen produc ...
Pyrococcus furiosus
Mol. Microbiol.
77
1111-1122
2010
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1
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1
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674514
Guiral
A membrane-bound multienzyme, ...
Aquifex aeolicus
J. Biol. Chem.
280
42004-42015
2005
1
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659878
Laska
Membrane-bound hydrogenase and ...
Acidianus ambivalens
Microbiology
149
2357-2371
2003
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1
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1
1
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437780
Keller
Hydrogen-sulfur oxidoreductase ...
Pyrodictium abyssi
Methods Enzymol.
331
442-451
2001
1
-
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4
1
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1
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437781
Laska
Improved purification of the m ...
Acidianus ambivalens
J. Chromatogr. B
737
151-160
2000
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437782
Ng
Purification and some properti ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans NASF-1
J. Biosci. Bioeng.
90
199-203
2000
-
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1
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6
1
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1
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2
1
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1
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439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
-
-
-
-
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-
-
-
1
2
5
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4
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1
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1
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1
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437773
Klimmek
The single cysteine residue of ...
Wolinella succinogenes
Eur. J. Biochem.
263
79-84
1999
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1
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1
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4
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745391
Silva
On the prosthetic groups of t ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
4
284-291
1999
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745603
Selvaggi
-
In vitro hydrogen photoproduc ...
Pyrococcus furiosus
J. Photochem. Photobiol. A
125
107-112
1999
-
1
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437778
Dirmeier
Purification and properties of ...
Pyrodictium abyssi
Eur. J. Biochem.
252
486-491
1998
1
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437779
Sugio
Purification and characterizat ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
62
705-709
1998
2
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7
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1
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1
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675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
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437776
Arendsen
Redox properties of the sulfhy ...
Pyrococcus furiosus
FEBS Lett.
368
117-121
1995
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2
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723118
Pedroni
Characterization of the locus ...
Pyrococcus furiosus
Microbiology
141
449-458
1995
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1
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437774
Fauque
-
Sulfur reductases from spirill ...
Sulfurospirillum deleyianum, Wolinella succinogenes
Methods Enzymol.
243
367-383
1994
3
-
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3
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2
2
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2
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1
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437775
Jankielewicz
-
Polysulfide reductase and form ...
Wolinella succinogenes
Arch. Microbiol.
162
238-242
1994
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