KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
reduced methyl viologen | - |
Enterobacter cloacae | |
0.72 | - |
reduced ferredoxin | - |
Enterobacter cloacae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Enterobacter cloacae | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains ca. 11.47 gm-atom Fe/mol, iron molecules exist as [4Fe-4S] clusters | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Enterobacter cloacae |
50800 | - |
1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
IIT-BT08 (MTCC 5373) | - |
Purification (Comment) | Organism |
---|---|
1284fold with a yield of 16.17%, by ultracentrifugation, ion exchange chromatography, gel filtration and hydrophobic-interaction chromatography | Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.26 | - |
crude extract | Enterobacter cloacae |
335 | - |
1284fold purified enzyme | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + reduced benzyl viologen | 58.8% relative activity, purified enzyme | Enterobacter cloacae | H2 + oxidized benzyl viologen | - |
? | |
H+ + reduced ferredoxin | 94.3% relative activity, purified enzyme | Enterobacter cloacae | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced methyl viologen | 100% relative activity, purified enzyme | Enterobacter cloacae | H2 + oxidized methyl viologen | - |
? | |
additional information | no activity of the purified enzyme with methylene blue, NADH, NADPH and Na-dithionite as electron-donor | Enterobacter cloacae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry | Enterobacter cloacae |
Synonyms | Comment | Organism |
---|---|---|
Fe-H2ase | - |
Enterobacter cloacae |
Fe-hydrogenase | - |
Enterobacter cloacae |
[Fe]-hydrogenase | - |
Enterobacter cloacae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Enterobacter cloacae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 65% of maximal activity, 50: about 80% of maximal activity | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | - |
Enterobacter cloacae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity | Enterobacter cloacae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterobacter cloacae | isoelectric focusing | - |
5.6 |