Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.7.2 extracted from

  • Dutta, T.; Das, A.; Das, D.
    Purification and characterization of [Fe]-hydrogenase from high yielding hydrogen-producing strain, Enterobacter cloacae IIT-BT08 (MTCC 5373) (2009), Int. J. Hydrogen Energy, 34, 7530-7537.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
reduced methyl viologen
-
Enterobacter cloacae
0.72
-
reduced ferredoxin
-
Enterobacter cloacae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Enterobacter cloacae 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains ca. 11.47 gm-atom Fe/mol, iron molecules exist as [4Fe-4S] clusters Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Enterobacter cloacae
50800
-
1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
IIT-BT08 (MTCC 5373)
-

Purification (Commentary)

Purification (Comment) Organism
1284fold with a yield of 16.17%, by ultracentrifugation, ion exchange chromatography, gel filtration and hydrophobic-interaction chromatography Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.26
-
crude extract Enterobacter cloacae
335
-
1284fold purified enzyme Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + reduced benzyl viologen 58.8% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized benzyl viologen
-
?
H+ + reduced ferredoxin 94.3% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized ferredoxin
-
?
H+ + reduced methyl viologen 100% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized methyl viologen
-
?
additional information no activity of the purified enzyme with methylene blue, NADH, NADPH and Na-dithionite as electron-donor Enterobacter cloacae ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry Enterobacter cloacae

Synonyms

Synonyms Comment Organism
Fe-H2ase
-
Enterobacter cloacae
Fe-hydrogenase
-
Enterobacter cloacae
[Fe]-hydrogenase
-
Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Enterobacter cloacae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 65% of maximal activity, 50: about 80% of maximal activity Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2
-
Enterobacter cloacae

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity Enterobacter cloacae

pI Value

Organism Comment pI Value Maximum pI Value
Enterobacter cloacae isoelectric focusing
-
5.6