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Literature summary for 1.12.7.2 extracted from
- Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H2-accumulation in Escherichia coli BL21(DE3) (2008), Appl. Microbiol. Biotechnol., 78, 853-862.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Clostridium acetobutylicum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | one mol of HydA contains 7 mol iron | Clostridium acetobutylicum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose 6 Fast Flow chromatography | Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
recombinant, purified enzyme | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin | under anoxic conditions | Clostridium acetobutylicum | H+ + reduced ferredoxin | - |
r | |
| H2 + oxidized methyl viologen | - |
Clostridium acetobutylicum | H+ + reduced methyl viologen | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fe-Fe hydrogenase | - |
Clostridium acetobutylicum |
| hydA | - |
Clostridium acetobutylicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | dependent | Clostridium acetobutylicum | |
| iron-sulfur centre | - |
Clostridium acetobutylicum | |
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