BRENDA - Enzyme Database show
show all sequences of 1.12.7.2

A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis

Soboh, B.; Linder, D.; Hedderich, R.; Microbiology 150, 2451-2463 (2004)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
reduced ferredoxin
70°C
Caldanaerobacter subterraneus subsp. tengcongensis
0.015
-
oxidized ferredoxin
70°C
Caldanaerobacter subterraneus subsp. tengcongensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane bound
Caldanaerobacter subterraneus subsp. tengcongensis
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
NiFe hydrogenase
Caldanaerobacter subterraneus subsp. tengcongensis
Ni
NiFe hydrogenase, contains 0.9 mol Ni per mol enzyme
Caldanaerobacter subterraneus subsp. tengcongensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14700
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
17000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
32000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
40000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
69000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
H+ + reduced ferredoxin
Caldanaerobacter subterraneus subsp. tengcongensis
-
H2 + oxidized ferredoxin
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-
Purification (Commentary)
Commentary
Organism
Q-sepharose HiLoad, hydroxyapatite, Superdex 200
Caldanaerobacter subterraneus subsp. tengcongensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38.5
-
reduction of metronidiazole
Caldanaerobacter subterraneus subsp. tengcongensis
391
-
reduction of methyl viologen
Caldanaerobacter subterraneus subsp. tengcongensis
1260
-
oxidation of methyl viologen
Caldanaerobacter subterraneus subsp. tengcongensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H+ + reduced ferredoxin
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H2 + oxidized ferredoxin
-
-
-
r
H+ + reduced methyl viologen
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H2 + oxidized methyl viologen
-
-
-
r
H2 + ferredoxin + oxidized metronidazole
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H+ + ferredoxin + reduced metronidazole
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
reduced ferredoxin
70°C
Caldanaerobacter subterraneus subsp. tengcongensis
0.015
-
oxidized ferredoxin
70°C
Caldanaerobacter subterraneus subsp. tengcongensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane bound
Caldanaerobacter subterraneus subsp. tengcongensis
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
NiFe hydrogenase
Caldanaerobacter subterraneus subsp. tengcongensis
Ni
NiFe hydrogenase, contains 0.9 mol Ni per mol enzyme
Caldanaerobacter subterraneus subsp. tengcongensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14700
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
17000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
32000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
40000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
69000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
H+ + reduced ferredoxin
Caldanaerobacter subterraneus subsp. tengcongensis
-
H2 + oxidized ferredoxin
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
Q-sepharose HiLoad, hydroxyapatite, Superdex 200
Caldanaerobacter subterraneus subsp. tengcongensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38.5
-
reduction of metronidiazole
Caldanaerobacter subterraneus subsp. tengcongensis
391
-
reduction of methyl viologen
Caldanaerobacter subterraneus subsp. tengcongensis
1260
-
oxidation of methyl viologen
Caldanaerobacter subterraneus subsp. tengcongensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H+ + reduced ferredoxin
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H2 + oxidized ferredoxin
-
-
-
r
H+ + reduced methyl viologen
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H2 + oxidized methyl viologen
-
-
-
r
H2 + ferredoxin + oxidized metronidazole
-
656734
Caldanaerobacter subterraneus subsp. tengcongensis
H+ + ferredoxin + reduced metronidazole
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE
Caldanaerobacter subterraneus subsp. tengcongensis
Other publictions for EC 1.12.7.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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4
2
1
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1
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-
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-
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-
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1
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1
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1
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742698
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1
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2
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1
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6
1
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1
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1
1
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2
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1
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6
1
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742784
Zheng
Hydrogen formation and its re ...
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J. Bacteriol.
196
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-
-
-
-
-
-
-
-
-
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1
-
4
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1
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742859
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289
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-
-
-
-
-
-
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1
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1
2
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5
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1
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4
1
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1
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1
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4
1
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1
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726335
Ghysels
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PLoS ONE
8
e64161
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-
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2
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5
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1
1
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724909
Silakov
-
Unraveling the electronic prop ...
Desulfovibrio desulfuricans
Eur. J. Inorg. Chem.
2011
1056-1066
2011
-
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1
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712301
Major
Characterization of energy-con ...
Methanococcus maripaludis
J. Bacteriol.
192
4022-4030
2010
-
-
-
-
-
-
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-
1
-
2
1
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2
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2
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1
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1
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2
1
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2
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1
1
-
-
-
712307
Welte
Function of Ech hydrogenase in ...
Methanosarcina mazei
J. Bacteriol.
192
674-678
2010
-
-
-
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-
1
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1
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1
1
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1
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1
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1
1
-
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1
1
-
-
-
727467
Welte
Involvement of Ech hydrogenase ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS J.
277
3396-3403
2010
-
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2
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5
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2
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4
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1
1
-
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-
701537
Marques
Purification, crystallization ...
Desulfovibrio vulgaris
Acta Crystallogr. Sect. F
65
920-922
2009
-
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-
1
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2
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2
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1
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1
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1
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704088
Dutta
-
Purification and characterizat ...
Enterobacter cloacae
Int. J. Hydrogen Energy
34
7530-7537
2009
-
-
-
-
-
-
-
2
1
1
2
-
-
1
-
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1
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2
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4
1
1
1
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1
1
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1
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2
1
1
2
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1
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2
-
4
1
1
1
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1
1
-
1
-
-
-
-
-
-
705334
Long
Hydrogenase/ferredoxin charge- ...
Chlamydomonas reinhardtii
J. Phys. Chem. A
113
4060-4067
2009
-
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6
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2
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1
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1
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1
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6
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1
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711817
Stripp
How algae produce hydrogen--ne ...
Chlamydomonas moewusii, Chlamydomonas reinhardtii, Chlorella fusca, Chlorococcum submarinum, Clostridium acetobutylicum, Clostridium pasteurianum, Desulfovibrio desulfuricans, Megasphaera elsdenii, Tetradesmus obliquus
Dalton Trans.
2009
9960-9969
2009
-
-
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-
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-
9
12
9
-
11
-
-
1
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10
1
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-
-
9
-
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9
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9
12
9
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1
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-
10
1
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1
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1
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712281
Schut
The iron-hydrogenase of Thermo ...
Thermotoga maritima
J. Bacteriol.
191
4451-4457
2009
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1
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1
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2
1
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684643
Akhtar
Deletion of iscR stimulates re ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
78
853-862
2008
-
-
1
-
-
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1
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3
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1
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1
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2
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2
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1
2
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685627
Long
Brownian dynamics and molecula ...
Chlamydomonas reinhardtii
Biophys. J.
95
3753-3766
2008
-
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1
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1
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686267
Guerrini
Characterization of two 2[4Fe4 ...
Clostridium acetobutylicum
Curr. Microbiol.
56
261-267
2008
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1
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1
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675496
Hedderich
Energy-converting [NiFe] hydro ...
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A multisubunit membrane-bound ...
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A novel type of iron hydrogena ...
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Characterization of hydrogenas ...
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Sapra
Purification and characterizat ...
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Enzymes of hydrogen metabolism ...
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Meuer
Purification and catalytic pro ...
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1999
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7
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439654
Ueno
Purification and characterizat ...
Alvikia littoralis
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443
144-148
1999
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1
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1
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1
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439656
Garcin
The crystal structure of a red ...
Desulfomicrobium baculatum
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1999
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439638
Fontecilla-Camps
Hydrogenase: a hydrogen-metabo ...
Desulfovibrio gigas
Biochimie
79
661-666
1997
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1
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439657
Romao
Characterization of the [NiFe] ...
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75-79
1997
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1
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Dobrindt
Purification and characterizat ...
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1
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2
4
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1
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1
-
3
1
1
-
-
1
1
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-
-
-
-
-
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439660
Fischer
Purification and characterizat ...
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1996
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1
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3
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1
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4
1
1
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1
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-
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439661
Yanke
Hydrogenase I of Clostridium p ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Anaerobe
1
61-67
1995
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2
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11
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4
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-
Purification and characterizat ...
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1994
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1
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Ma
Hydrogenase of the hyperthermo ...
Pyrococcus furiosus
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Happe
Isolation, characterization an ...
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Bryant
Characterization of hydrogenas ...
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George
X-ray-absorption-spectroscopic ...
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Macor
An investigation of hydrogenas ...
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6
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439670
Adams
-
The purification of hydrogenas ...
Clostridium pasteurianum
Biochim. Biophys. Acta
766
51-61
1984
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439671
Chen
Purification and properties of ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Biochem. Biophys. Res. Commun.
122
9-16
1984
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11
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439672
Roessler
Purification of hydrogenase fr ...
Chlamydomonas reinhardtii 137 C(+), Chlamydomonas reinhardtii
Plant Physiol.
75
705-709
1984
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2
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4
1
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6
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439673
Schneider
Purification of hydrogenases b ...
Clostridium pasteurianum, Clostridium pasteurianum MR 505, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochem. J.
213
391-398
1983
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45
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439674
Van Dijk
The effects of pH and redox po ...
Megasphaera elsdenii
Eur. J. Biochem.
114
209-219
1981
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439675
Khan
The effect of electron carrier ...
Clostridium pasteurianum
Biochim. Biophys. Acta
659
457-465
1981
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1
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439676
Van Dijk
Properties of the hydrogenase ...
Megasphaera elsdenii
Eur. J. Biochem.
107
251-261
1980
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6
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1
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439677
Van Dijk
Purification and properties of ...
Megasphaera elsdenii, Megasphaera elsdenii LC1
Eur. J. Biochem.
102
317-330
1979
3
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439678
Klibanov
Chelating agents protect hydro ...
Clostridium pasteurianum
Biochim. Biophys. Acta
547
411-416
1979
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1
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1
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439679
Chen
Isolation and properties of a ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Biochem. Biophys. Res. Commun.
84
1144-1150
1978
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10
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439680
Klibanov
A rationale for stabilization ...
Clostridium pasteurianum
Proc. Natl. Acad. Sci. USA
75
3640-3643
1978
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1
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439681
Erbes
The kinetics of methyl viologe ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Biochim. Biophys. Acta
525
45-54
1978
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8
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439682
Chen
Purification and properties of ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Biochim. Biophys. Acta
371
283-298
1974
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8
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439683
Thauer
The reaction of the iron-sulfu ...
Clostridium pasteurianum
Eur. J. Biochem.
42
447-452
1974
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1
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439684
Nakos
Structural properties of hydro ...
Clostridium pasteurianum, Clostridium pasteurianum W5
Biochemistry
10
2442-2449
1971
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1
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