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Literature summary for 1.12.7.2 extracted from
- Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii (1993), Eur. J. Biochem., 214, 475-481.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
oxidized ferredoxin | - |
Chlamydomonas reinhardtii |  |
| 0.83 | - |
methyl viologen | - |
Chlamydomonas reinhardtii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 4 atoms per mol enzyme | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
SDS- and native PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin | Chlamydomonas reinhardtii | - |
H+ + reduced ferredoxin | - |
r | |
| H2 + oxidized ferredoxin | Chlamydomonas reinhardtii 137 C(+) | - |
H+ + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
| Chlamydomonas reinhardtii 137 C(+) | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapid inactivation under aerobic conditions | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 935 | - |
hydrogen production, reduced methyl viologen as electron donor | Chlamydomonas reinhardtii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, several weeks, strictly anaerobical storage | Chlamydomonas reinhardtii |
| 5°C, 5 d, strictly anaerobical storage | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H+ + reduced methyl viologen | - |
Chlamydomonas reinhardtii | H2 + oxidized methyl viologen | - |
? | |
| H+ + reduced methyl viologen | - |
Chlamydomonas reinhardtii 137 C(+) | H2 + oxidized methyl viologen | - |
? | |
| H2 + electron acceptor | methyl viologen as electron acceptor | Chlamydomonas reinhardtii | H+ + reduced electron acceptor | - |
r | |
| H2 + electron acceptor | methyl viologen as electron acceptor | Chlamydomonas reinhardtii 137 C(+) | H+ + reduced electron acceptor | - |
r | |
| H2 + oxidized ferredoxin | - |
Chlamydomonas reinhardtii | H+ + reduced ferredoxin | - |
r | |
| H2 + oxidized ferredoxin | - |
Chlamydomonas reinhardtii 137 C(+) | H+ + reduced ferredoxin | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
hydrogen evolution | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
hydrogen evolution | Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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