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Literature summary for 1.12.7.2 extracted from

  • Sapra, R.; Verhagen, M.F.; Adams, M.W.
    Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (2000), J. Bacteriol., 182, 3423-3428.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.7.2

Inhibitors

Inhibitors Comment Organism Structure
O2 50% loss of H2 evolution activity after 3 h Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pyrococcus furiosus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 4 Fe-atoms per mol hydrogenase Pyrococcus furiosus
Ni 1 Ni atom per mol, redox active Ni-site Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus
40000
-
 alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

Oxidation Stability Organism
rapid inactivation under aerobic conditions Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions, solubilized with n-dodecyl-beta-D-maltoside Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
 hydrogen oxidation, benzyl viologen as electron acceptor Pyrococcus furiosus
9.4
-
 hydrogen evolution, reduced methyl viologen as electron donor Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + electron acceptor methyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
H2 + electron acceptor benzyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
H2 + electron acceptor NADP+ as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
additional information ferredoxin is not an efficient electron carrier for both hydrogenases Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
dimer alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 50% inactivation after 30 min Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pyrococcus furiosus