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Literature summary for 1.12.5.1 extracted from
- Hydrogen-ubiquinone oxidoreductase activity by the Bradyrhizobium japonicum membrane-bound hydrogenase (1993), FEMS Microbiol. Lett., 110, 257-264.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-n-heptyl-4-hydroxyquinoline-N-oxide | 0.1 mM, 17% inhibition | Bradyrhizobium japonicum |  |
| antimycin A | 0.1 mM, 47% inhibition | Bradyrhizobium japonicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | a nickel-iron hydrogenase | Bradyrhizobium japonicum | |
| Ni | a nickel-iron hydrogenase | Bradyrhizobium japonicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 65000 + 1 * 33000 | Bradyrhizobium japonicum |
| 65000 | - |
1 * 65000 + 1 * 33000 | Bradyrhizobium japonicum |
| 82600 | - |
gel filtration | Bradyrhizobium japonicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium japonicum | - |
expresses hydrogenase constitutively | - |
| Bradyrhizobium japonicum SR473 | - |
expresses hydrogenase constitutively | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bradyrhizobium japonicum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 65000 + 1 * 33000 | Bradyrhizobium japonicum |
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