Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.1.5 extracted from

  • Chandrayan, S.K.; McTernan, P.M.; Hopkins, R.C.; Sun, J.; Jenney, F.E.; Adams, M.W.
    Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase (2012), J. Biol. Chem., 287, 3257-3264.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.1.5

Application

Application Comment Organism
synthesis the recombinant enzyme is efficient in vitro biohydrogen production Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 1 nickel-iron catalytic site and 6 iron-sulfur clusters, contains 23 iron atoms/heterotetramer Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus COM1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-tag II column chromatography Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23
-
 crude native enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus
7.96
-
 crude recombinant enzyme, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus
126
-
 recombinant enzyme after 24fold purification, using methyl viologen as cosubstrate, at pH 8.4 and 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + NADPH
-
 Pyrococcus furiosus H2 + NADP+
-
 r
H+ + NADPH
-
 Pyrococcus furiosus COM1 H2 + NADP+
-
 r
H+ + reduced methyl viologen
-
 Pyrococcus furiosus H2 + oxidized methyl viologen
-
 ?
H+ + reduced methyl viologen
-
 Pyrococcus furiosus COM1 H2 + oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
heterotetramer
-
 Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
SHI
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 90 the native enzyme shows half-lives of 14 h at 90°C under argon (H2 evolution), 21 h at 25°C under air (H2 evolution), and 10 h at 90°C under argon (H2 oxidation). The recombinant enzyme shows half-lives of 6 h at 90°C under argon (H2 evolution), 25 h at 25°C under air (H2 evolution), and 5 h at 90°C under argon (H2 oxidation) Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
flavin
-
 Pyrococcus furiosus
NADPH dependent on Pyrococcus furiosus
[2Fe-2S]-center contains 1 [2Fe-2S] centre Pyrococcus furiosus
[4Fe-4S]-center contains 2 [4Fe-4S] centres Pyrococcus furiosus