BRENDA - Enzyme Database
show all sequences of 1.12.1.4

Importance of hydrogen bonding in fine tuning the [2Fe-2S] cluster redox potential of HydC from Thermotoga maritima

Birrell, J.A.; Laurich, C.; Reijerse, E.J.; Ogata, H.; Lubitz, W.; Biochemistry 55, 4344-4355 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Thermotoga maritima
Engineering
Amino acid exchange
Commentary
Organism
A85P
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
V131N
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima
-
H+ + NADH + reduced ferredoxin
-
-
?
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
H+ + NADH + reduced ferredoxin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
Purification (Commentary)
Commentary
Organism
streptactin column chromatography
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima
H+ + NADH + reduced ferredoxin
-
-
-
?
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
H+ + NADH + reduced ferredoxin
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Thermotoga maritima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Thermotoga maritima
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A85P
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
V131N
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima
-
H+ + NADH + reduced ferredoxin
-
-
?
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
H+ + NADH + reduced ferredoxin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
streptactin column chromatography
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima
H+ + NADH + reduced ferredoxin
-
-
-
?
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
H+ + NADH + reduced ferredoxin
-
-
-
?
Other publictions for EC 1.12.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741931
Birrell
Importance of hydrogen bondin ...
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
Biochemistry
55
4344-4355
2016
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741708
Maso
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A conformational study of the ...
Thermotoga neapolitana
Appl. Magn. Reson.
46
465-479
2015
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1
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1
1
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740625
Zheng
Hydrogen formation and its reg ...
Ruminococcus albus, Ruminococcus albus DSM 20455
J. Bacteriol.
196
3840-3852
2014
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3
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2
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1
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3
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1
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1
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2
1
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742697
Albertini
-
The proton iron-sulfur cluste ...
Thermotoga neapolitana
Int. J. Hydrogen Energy
39
18574-18582
2014
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1
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1
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1
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740618
Wang
A reversible electron-bifurcat ...
Moorella thermoacetica
J. Bacteriol.
195
1267-1275
2013
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2
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1
4
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1
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1
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3
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4
1
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4
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4
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2
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1
4
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3
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1
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724434
Berto
The [4Fe-4S]-cluster coordinat ...
Thermotoga neapolitana
Biochim. Biophys. Acta
1817
2149-2157
2012
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1
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5
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712281
Schut
The iron-hydrogenase of Thermo ...
Thermotoga maritima, Thermotoga maritima DSM 3109
J. Bacteriol.
191
4451-4457
2009
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1
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2
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11
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3
1
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1
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716106
Verhagen
Fe-only hydrogenase from Therm ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Methods Enzymol.
331
216-226
2001
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8
2
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8
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6
1
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